钙离子拮抗剂SKF96365与LOE908对缺血再灌注损伤的心肌内皮素-1表达的影响

发布时间：2018-03-03 03:13

本文选题：内皮素-　切入点：SKF　出处：《广东医学》2017年13期 　论文类型：期刊论文

【摘要】：目的探讨两种钙离子拮抗剂SKF96365和LOE908对缺血再灌注损伤的兔心肌内皮素-1(endothelin,ET-1)表达的影响。方法健康雄性成年新西兰家兔32只,随机分为4组(n=8):假手术对照(Sham)组、心肌缺血再灌注(I/R)组、SKF96365(SKF)组和LOE908组。开胸后心脏适应10 min,Sham组用4-0丝线仅穿过而不结扎冠脉左前降支上1/3处30 min,再观察120 min;I/R组用4-0丝线结扎冠脉左前降支上1/3处30 min,再恢复灌注120 min;SKF组和LOE908组在I/R组基础上,于恢复灌注即刻分别静脉注射0.06 mg/kg SKF96365和0.1 mg/kg LOE908,4组家兔于再灌注后30、60、120 min检测静脉血ET-1浓度变化,比较各组静脉血乳酸脱氢酶(LDH)、肌酸激酶(CK-MB)含量,检测心肌梗死面积。结果与Sham组相比,I/R组、SKF组和LOE908组静脉血ET-1浓度随心肌再灌注时间延长而上升(P0.05);与I/R组相比,SKF组和LOE908组中LDH与CKMB水平均降低(P0.05),梗死面积减少(P0.05);与LOE908组比较,SKF组再灌注120 min后LDH、CK-MB水平及心肌梗死面积均显著减少(P0.05)。结论静脉血ET-1随心肌再灌注时间的延长而升高,而SKF96365和LOE908这两种钙离子拮抗剂能抑制其表达,而在心肌再灌注120 min时,SKF96365对缺血再灌注损伤心肌的保护作用比LOE908更显著。
[Abstract]:Objective to investigate the effects of two calcium antagonists, SKF96365 and LOE908, on the expression of endothelin-1 endothelin 1 (et 1) in rabbit myocardium after ischemia reperfusion injury. Methods Thirty-two healthy male New Zealand rabbits were randomly divided into 4 groups: sham-operated control group. Myocardial ischemia reperfusion I / R group (SKF96365) group and LOE908 group. 10 minutes after thoracotomy, the heart adapts to the heart in the sham group with 4-0 silk thread only through the left anterior descending coronary artery for 30 minutes, and then the 120min I / R group ligated the superior left anterior descending coronary artery with 4-0 silk line for 30 minutes, and the left anterior descending coronary artery was ligated with 4-0 silk line for 30 minutes, and the left anterior descending coronary artery was ligated with 4-0 silk line for 30 minutes, then the left anterior descending coronary artery was ligated with 4-0 wire for 30 minutes. At 120min after reperfusion, SKF and LOE908 groups were on the basis of I / R group. The concentration of ET-1 in venous blood was detected by intravenous injection of 0. 06 mg/kg SKF96365 and 0. 1 mg/kg LOE908 + #number0# min immediately after reperfusion, and the contents of lactate dehydrogenase (LDH) and creatine kinase (CK-MBB) were compared in each group. Results compared with the Sham group, the venous ET-1 concentration in the I / R group and the LOE908 group increased with the prolongation of myocardial reperfusion time (P 0.05), and the levels of LDH and CKMB in the I / R group and the LOE908 group were lower than those in the I / R group, and the infarct area was decreased (P 0.05). Compared with LOE908 group, the level of LDH- CK-MB and myocardial infarction size decreased significantly in SKF group after reperfusion for 120 min. Conclusion the level of ET-1 in venous blood increases with the prolongation of myocardial reperfusion time. SKF96365 and LOE908, two calcium antagonists, inhibited their expression, and the protective effect of SKF96365 on ischemia-reperfusion injury was more significant than that of LOE908 at 120 min reperfusion.
【作者单位】：广东省第二人民医院麻醉科;广东省人民医院(广东省医学科学院)麻醉科;
【分类号】：R54

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