载脂蛋白J在大鼠颈动脉再狭窄模型中表达规律的研究

发布时间：2018-03-03 06:21

本文选题：载脂蛋白J类　切入点：颈动脉狭窄　出处：《中国循环杂志》2017年12期 　论文类型：期刊论文

【摘要】：目的:研究载脂蛋白J(Apo J)在大鼠颈动脉球囊扩张后血管再狭窄模型中的表达规律。方法:40只Wistar大鼠随机分为两组:实验组和对照组,每组20只。采用2F Fogarty球囊导管于右侧颈总动脉进行球囊拉伤造模,术后1、2、3、4周共四个时间点,每组每个时间点5只大鼠。在各时间点,取右侧颈总动脉后,采用苏木伊红染色法(HE)观察其形态学变化,免疫组化检测Apo J蛋白表达,实时定量聚合酶链式反应(qRT-PCR)检测Apo J基因的表达水平。结果:与对照组各时间点相比,实验组在相同时间点内膜增生明显,Apo J蛋白及基因表达明显上调(P0.05);且实验组随着损伤时间延长,内膜持续增生至第四周达到最大值,Apo J蛋白及基因表达在第3周达到高峰,第4周有所下降(P0.05)。结论:Apo J可能与血管损伤及损伤后内膜增生密切相关,血管中高表达的Apo J主要来源于损伤组织局部的血管平滑肌细胞(VSMC)合成,推测它可能是损伤后的一种代偿,通过抑制VSMC的增值和迁移的活性对再狭窄起保护作用。为寻找新的经皮冠状动脉介入治疗术后血管再狭窄的干预提供新的靶点。
[Abstract]:Objective: to study the expression of apolipoprotein (apo J) in carotid artery restenosis after balloon dilatation in rats. Methods: 40 Wistar rats were randomly divided into two groups: experimental group and control group. 20 rats in each group were used to model the right common carotid artery by using 2F Fogarty balloon catheter. There were four time points at 1, 2 and 3 weeks after operation, and 5 rats in each group. At each time point, the right common carotid artery was taken out, and the right common carotid artery was taken from the right common carotid artery at each time point, and the right common carotid artery was taken from the right common carotid artery at each time point. The morphological changes were observed by hematoxylin staining, the expression of Apo J protein was detected by immunohistochemistry, and the expression level of Apo J gene was detected by real-time quantitative polymerase chain reaction (QRT). Results: compared with the control group, the expression of Apo J gene was detected at different time points. In the experimental group, the expression of Apo J protein and gene increased significantly at the same time point, and the expression of Apo J protein and gene reached the peak at the third week with the extension of injury time. Conclusion the high expression of Apo J may be related to vascular injury and intimal hyperplasia after injury. The high expression of Apo J is mainly derived from the synthesis of vascular smooth muscle cells (VSMC). It is speculated that it may be a kind of compensation after injury, which can protect restenosis by inhibiting the proliferation and migration activity of VSMC, and provide a new target for finding a new intervention of percutaneous coronary intervention in the treatment of restenosis after percutaneous coronary intervention.
【作者单位】：天津市胸科医院心内科;
【基金】：天津市卫计委重点攻关项目(13KG131);天津市卫计委科技基金重点项目(2015KR07)
【分类号】：R-332;R541.4

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