细胞间黏附分子-1与小鼠新生血管的相关性研究

发布时间：2018-03-08 11:31

本文选题：动脉粥样硬化　切入点：细胞间黏附分子-　出处：《中国临床药理学杂志》2017年14期 　论文类型：期刊论文

【摘要】：目的研究细胞间黏附分子-1(ICAM-1)和新生血管在动脉粥样硬化中的表达情况的相关性研究。方法 8周龄Apo E-/-小鼠给予高脂饮食喂养分别至16,24,32周龄,建立代表动脉粥样硬化病变自起始至脂肪纹、纤维斑块及粥样斑块形成不同病变阶段的实验动物模型,取小鼠血清,全自动生化分析仪测定总胆固醇(TC)、低密度脂蛋白胆固醇(LDL)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL),通过免疫组化的方法检测动脉粥样硬化斑块内ICAM-1的表达量。用CD31抗体免疫组化标记新生血管。结果随着周龄增加,TC和LDL逐步增加,8,16,24,32周龄小鼠TC分别为(1.48±0.13),(3.56±0.25),(8.95±0.33),(12.23±0.58)mmol·L~(-1),LDL分别为(0.65±0.06),(2.36±0.18),(4.19±0.31),(5.64±0.55)mmol·L~(-1),与8周龄相比,差异均有统计学意义(P0.05)。ICAM相对表达量随周龄逐步增加,8,16,24,32周龄小鼠ICAM-1分别为0.05±0.02,1.25±0.35,2.56±0.46,5.23±0.17;8,16,24,32周龄小鼠新生血管数分别为2.12±0.56,7.17±2.64,12.86±3.72,16.78±3.52,与8周龄相比,差异均有统计学意义(P0.05)。ICAM-1与新生血管呈正相关(y=2.79 x+3.40,r2=0.927 9)。结论动脉粥样进展过程中,ICAM-1与新生血管表达量逐步增加,ICAM-1的表达增加可能是导致动脉粥样新生血管增加的原因。
[Abstract]:Objective to study the relationship between the expression of intercellular adhesion molecule-1 (ICAM-1) and the expression of neovascularization in atherosclerosis. Methods 8-week-old Apo E-r-mice were fed with a high-fat diet until 16 ~ 24 ~ 32 weeks old. To establish experimental animal models representing the different stages of atherosclerotic lesions from the beginning to the fatty stria, fiber plaques and atherosclerotic plaques, and to take the serum of mice. Total cholesterol (TC), low density lipoprotein cholesterol (LDL), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) were measured by automatic biochemical analyzer. The expression of ICAM-1 in atherosclerotic plaques was detected by immunohistochemical method. Results with the increase of age, TC and LDL were 1.48 卤0.13, 3.56 卤0.33 and 12.23 卤0.58 mmol 路L ~ (-1), respectively, in mice of 32 weeks old, 0.65 卤0.06 ~ (2.36 卤0.18) 卤4.19 卤0.31 ~ (mmol 路L ~ (-1)), respectively, as compared with those of 8 weeks of age. There were significant differences in the relative expression of P0.05A and ICAM with the increase of age. The ICAM-1 of the mice at the age of 8 weeks was 0.05 卤0.02, 1.25 卤0.35, 2.56 卤0.465.23 卤0.1765.23 卤0.1767.17 卤2.67.17 卤2.612.86 卤3.72 卤3.52, respectively, compared with that of the 8-week old mice, the number of neovascularization was 2.12 卤0.567.17 卤2.6412.86 卤3.72 卤3.52, respectively, compared with that of the 8-week-old mice, the number of neovascularization was 2.12 卤0.567.17 卤2.6412.86 卤3.72 卤3.52, respectively. There was a positive correlation between ICAM-1 and neovascularization. Conclusion the increased expression of ICAM-1 and neovascularization may be the cause of the increase of angiogenesis.
【作者单位】：余杭区第一人民医院心血管内科;
【基金】：杭州市科技局基金资助项目(20150633B67)
【分类号】：R543.5

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