酒精性心肌病模型大鼠缝隙连接蛋白异常表达的机制

发布时间：2018-03-08 13:05

本文选题：酒精性心肌病　切入点：缝隙连接蛋白　出处：《中国老年学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨缝隙连接蛋白(Cx)异常表达在酒精性心肌病大鼠发病中的作用。方法 50只清洁级SD大鼠随机分为对照组、模型组、U0126低剂量、中剂量和高剂量组(n=10)。采用4.0%乙醇(1次/d,持续12 w)复制酒精性心肌病模型,分析U0126治疗后各组大鼠心功能变化及Cx的表达变化。结果酒精性心肌病大鼠心功能及左心室指数和心脏指数存在明显异常,且U0126能够有效改善大鼠心功能和异常的心脏指数(P0.05);血清Cx40、Cx43和Cx45蛋白表达明显降低(P0.01),而Ras、Raf、MEK、ERK1/2、基质金属蛋白酶(MMP)2、MMP9和MMP10的表达明显升高,U0126能有效恢复上述蛋白的异常表达(P0.05)。结论大鼠酒精性心肌病的发病过程与Cx及MMPs异常表达有关,且可能是药物治疗的靶点。
[Abstract]:Objective to investigate the role of the abnormal expression of gap junction protein (CX) in the pathogenesis of alcoholic cardiomyopathy in rats. Methods Fifty SD rats of clean grade were randomly divided into control group, model group and low dose of U0126. The model of alcoholic cardiomyopathy was established in the middle and high dose groups. The model was established by using 4.0% ethanolone once a day for 12 weeks. The changes of cardiac function and the expression of CX were analyzed after U0126 treatment. Results there were significant abnormalities in cardiac function, left ventricular index and cardiac index in rats with alcoholic cardiomyopathy. U0126 could effectively improve the cardiac function and abnormal cardiac index (P0.05), and the expression of Cx40Cx43 and Cx45 decreased significantly, while the expression of Ras Rafn MEKERK1 / 2, matrix metalloproteinase (MMP-MMP2) MMP9 and MMP10 could effectively recover the abnormal expression of U0126. Conclusion the pathogenesis of alcoholic cardiomyopathy is related to the abnormal expression of CX and MMPs. And may be a target for drug therapy.
【作者单位】：遵义医学院第一附属医院心血管内科;
【基金】：贵州省科学技术基金资助(No.20132326)
【分类号】：R542.2;R-332

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