Ad-HIF-1α双突变体对急性心肌梗死大鼠心功能的短期影响

发布时间：2018-03-10 20:34

本文选题：Ad-HIF-α　切入点：心肌梗死　出处：《广东医学》2017年07期 　论文类型：期刊论文

【摘要】：目的研究重组腺病毒低氧诱导因子-1α双突变体基因(Ad-HIF-1α-564/402)对心肌梗死大鼠心功能的影响。方法 36只雄性SD大鼠建立急性心肌梗死模型,随机分成3组,分别为生理盐水组(Saline组,n=12)、腺病毒空载体组(Ad-Null组,n=12)、Ad-HIF-1α-564/402组(n=12),分别在术后即刻肌肉注射0.1 m L生理盐水、Ad-Null、Ad-HIF-1α-564/402,病毒滴度1.0×10~8PFU。术后7d,行心脏超声检查,了解心功能情况;检测血清中肌酸激酶同工酶(CK-MB)、肌钙蛋白I(c Tn I)含量;氯化三苯基四氮唑法(TTC)评价心肌梗死面积。结果基因转染后7d,心脏超声结果显示,Ad-HIF-1α-564/402组心功能指标中左室射血分数(LVEF)、心输出量(CO)优于Saline组和Ad-Null组,3组间差异有统计学意义(P0.05),Saline组和Ad-Null组差异无统计学意义(P0.05),Ad-HIF-1α-564/402组左室短轴缩短率(LVFS)大于其他两组,但3组间差异无统计学意义(P0.05);CK-MB、c Tn I在Ad-HIF-1α-564/402组含量较其他两组为低,3组间差异有统计学意义(P0.05),Saline组和Ad-Null组差异无统计学意义(P0.05);TTC法评价心肌梗死面积,结果示AdHIF-1α-564/402组心肌梗死面积百分数小于其他两组,3组间差异有统计学意义(P0.05),Saline组和AdNull组差异无统计学意义(P0.05)。结论 Ad-HIF-1α-564/402能减轻心肌梗死大鼠近期心肌损伤,减小梗死面积,改善心功能。
[Abstract]:Objective to study the recombinant adenovirus of hypoxia inducible factor alpha -1 double mutant gene (Ad-HIF-1 alpha -564/402) on cardiac function in myocardial infarction rats model of acute myocardial infarction. Methods 36 male SD rats were randomly divided into 3 groups, respectively, the normal saline group (group Saline, n=12), adenovirus vector group (group Ad-Null, n=12), Ad-HIF-1 -564/402 group (n=12), respectively, in the immediate postoperative intramuscular injection of 0.1 M normal saline L, Ad-Null, Ad-HIF-1 alpha -564/402, virus titer was 1 * 10~8PFU. 7d after surgery, cardiac ultrasound examination, the heart function; serum creatine kinase isoenzyme (CK-MB), cardiac troponin I (C Tn I) content; three phenyl tetrazolium chloride method (TTC) to evaluate the area of myocardial infarction. Results after transfection of 7D, echocardiography showed that Ad-HIF-1 alpha -564/402 group heart function index of left ventricular ejection fraction (LVEF), cardiac output (CO) is better than that of group Saline and Ad-Nul In l group, there were statistically significant differences between the 3 groups (P0.05), no statistically significant difference between Saline group and Ad-Null group (P0.05), Ad-HIF-1 -564/402 group left ventricular fractional shortening (LVFS) than the other two groups, but there was no significant difference between the 3 groups (P0.05); CK-MB C, Tn I in Ad-HIF-1 a -564/402 group was lower than the other two groups, there were statistically significant differences between the 3 groups (P0.05), no statistically significant difference between Saline group and Ad-Null group (P0.05); myocardial infarction area was evaluated with TTC, the results showed that AdHIF-1 alpha myocardial infarct area of -564/402 group was less than the percentage of his two group, there were significant differences between the 3 groups between (P0.05), no statistically significant difference between Saline group and AdNull group (P0.05). Conclusion Ad-HIF-1 alpha -564/402 can reduce myocardial infarction rats in myocardial injury, reduce infarct size and improve cardiac function.

【作者单位】：广州医科大学附属第四医院心血管内科;
【基金】：国家自然科学基金资助项目(编号:81300086) 广东省自然科学基金资助项目(编号:2015A030313451)
【分类号】：R542.22

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