可溶性环氧化物水解酶抑制剂在TGF-β1诱导的人脐静脉内皮细胞内皮-间质转化中的作用

发布时间：2018-03-12 20:29

  本文选题：可溶性环氧化物水解酶抑制剂　切入点：内皮-间质转化　出处：《郑州大学学报(医学版)》2017年02期 　论文类型：期刊论文

【摘要】：目的:观察可溶性环氧化物水解酶抑制剂t-AUCB对TGF-β1诱导的人脐静脉内皮细胞(HUVEC)内皮-间质转化(End MT)的影响。方法:采用t-AUCB(50μmol/L)预处理HUVEC 40 min后,用TGF-β1(10μg/L)诱导HUVEC 24 h,采用Western blot法检测Smad2/3的磷酸化水平;诱导72 h后,光镜观察细胞形态的变化,采用CCK-8试剂盒检测细胞活性,采用实时荧光定量PCR检测内皮细胞标记物(CD31)、间质细胞标记物(collagenⅠ、collagenⅢ、vimentin)及End MT中重要的下游转录因子(snail1、twist1、twist2、ZEB1)mRNA的表达。同时设TGF-β1和tAUCB单独作用组。结果:光镜下,TGF-β1组细胞转变为狭长形,细胞间隙疏松,TGF-β1+t-AUCB组、t-AUCB组细胞形态正常。TGF-β1组细胞CD31 mRNA表达下调,collagenⅠ、collagenⅢ、vimentin mRNA表达上调,snail1、twist1、twist2、ZEB1 mRNA表达上调,Smad2及Smad3蛋白磷酸化水平升高(P0.05)。与TGF-β1组比较,TGF-β1+t-AUCB组、t-AUCB组上述指标的表达均有一定程度的逆转(P0.05)。结论:t-AUCB可通过抑制内皮细胞的End MT而发挥抗纤维化作用。
[Abstract]:Aim: to observe the effect of t-AUCB, a soluble epoxide hydrolase inhibitor, on endothelium-mesenchymal transformation of human umbilical vein endothelial cells (HUVECs) induced by TGF- 尾 1. Methods: HUVEC was pretreated with t-AUCB50 渭 mol / L for 40 min. HUVEC was induced by TGF- 尾 1 10 渭 g / L for 24 h, and the phosphorylation level of Smad2/3 was detected by Western blot assay. After 72 h of induction, the changes of cell morphology were observed under light microscope, and the cell activity was detected by CCK-8 kit. Real time fluorescence quantitative PCR was used to detect the mRNA expression of endothelial cell marker CD31, interstitial cell marker collagen 鈪，

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