Tim-3通过胆固醇酯水解酶影响巨噬细胞泡沫化过程的机制

发布时间：2018-03-16 18:26

本文选题：胆固醇酯水解酶　切入点：巨噬细胞　出处：《中国老年学杂志》2017年15期 　论文类型：期刊论文

【摘要】：目的观察Tim-3是否通过调节巨噬细胞内胆固醇脂水解酶(CEH)的表达干扰泡沫化细胞的形成,进而影响动脉粥样硬化的发生发展。方法 Western印迹检测CEH蛋白的表达;液体闪烁计数测定THP-1巨噬细胞内胆固醇流出情况;HPLC检测胞内脂质含量;油红O染色显示胞内脂滴情况;ELISA检测肿瘤坏死因子(TNF)-α、MIF、白细胞介素(IL)-10的分泌情况。结果 Ad-Tim-3组细胞中CEH的表达被明显抑制,胞内胆固醇流出减少,胞内总胆固醇(TC)、胆固醇酯(CE)及游离胆固醇(FC)的含量明显增加,TNF-α和MIF分泌增多,IL-10的分泌减少;Ad-CEH组和Ad-Tim-3+Ad-CEH组细胞中CEH的表达水平被明显增强,胞内胆固醇流出增加,胞内TC、CE及FC的含量明显减少,TNF-α和MIF分泌减少,IL-10分泌增多。结论 Tim-3可以通过增强巨噬细胞内CEH的活性抑制巨噬细胞向泡沫细胞的转化。
[Abstract]:Objective to observe whether Tim-3 interferes with the formation of foamed cells by regulating the expression of cholesterol hydrolase in macrophages and thus affects the development of atherosclerosis. Methods the expression of CEH protein was detected by Western blot. Liquid scintillation count was used to detect cholesterol efflux in THP-1 macrophages. Oil red O staining showed that the intracellular lipid droplets were detected by Elisa to detect the secretion of TNF- 伪 and IL-IL-10. Results the expression of CEH was significantly inhibited and the intracellular cholesterol efflux was decreased in Ad-Tim-3 group. The contents of TC- 伪 and MIF in the cells of the Ad-CEH group and the Ad-Tim-3 Ad-CEH group were significantly increased, and the intracellular cholesterol efflux was increased, and the expression of CEH in the cells of the Ad-CEH group and the Ad-Tim-3 Ad-CEH group was significantly increased, and the increase of TNF- 伪 and MIF secretion was observed in the cells of the Ad-CEH group and the Ad-Tim-3 Ad-CEH group. The contents of TCN CE and FC significantly decreased the secretion of TNF- 伪 and MIF and decreased the secretion of IL-10. Conclusion Tim-3 can inhibit the transformation of macrophages to foam cells by enhancing the activity of CEH in macrophages.
【作者单位】：锦州医科大学附属第一医院;
【基金】：国家自然科学基金(81541099) 辽宁省自然科学基金(2016010330-301)
【分类号】：R54

【相似文献】