Irbesartan对冠状动脉粥样硬化小鼠的治疗作用及可能机制

发布时间：2018-03-19 22:31

本文选题：厄贝沙坦　切入点：动物模型　出处：《中国免疫学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:明确Irbesartan对冠状动脉粥样硬化小鼠的治疗作用,并进一步分析可能的机制。方法:将16只雄性Apo E-/-小鼠给予高脂饮食(1.25%胆固醇,10%脂肪)构建冠心病模型。模型建立后将小鼠随机分为治疗组[Irbesartan,50mg/(kg·d),4周]及对照组(等量生理盐水灌胃对照)。采用HE、ELISA、Western blot及免疫荧光技术分析疗效及机制。结果:治疗组小鼠动脉粥样斑块面积显著低于对照组(P0.05)。治疗组血管内IL-1β、IL-6及TNF-α水平显著低于对照组(P0.05)。治疗组小鼠血管周围脂肪组织内PPAR-γ及Adiponectin水平显著升高,而Leptin水平显著降低,与对照组相比差异具有统计学意义(P0.05)。Western blot及免疫荧光证实,Irbesartan显著抑制了治疗组小鼠血管周围脂肪组织内的NF-κB信号通路。结论:Irbesartan通过调节血管周围脂肪组织内PPAR-γ-NF-κb(p65)信号通路,调节血管周围脂肪组织功能及炎症,从而发挥抗动脉粥样硬化疗效。
[Abstract]:Objective: to investigate the therapeutic effect of Irbesartan on mice with coronary atherosclerosis. Methods: 16 male Apo E-r-mice were given 1.25% cholesterol 10% fat in a high-fat diet to establish a coronary heart disease model. After the establishment of the model, the mice were randomly divided into two groups: treatment group [Irbesartanan 50mg / kg / kg / d + 4 weeks] and control group (et al.). The therapeutic effect and mechanism were analyzed by Western blot and immunofluorescence technique. Results: the area of atherosclerotic plaques in the treatment group was significantly lower than that in the control group (P 0.05). The levels of IL-1 尾 -IL-6 and TNF- 伪 in the treatment group were significantly lower than those in the control group. The levels of PPAR- 纬 and Adiponectin in perivascular adipose tissue of mice in the treatment group were significantly higher than those in the control group. However, the level of Leptin decreased significantly, The results showed that Irbesartan significantly inhibited NF- 魏 B signaling pathway in perivascular adipose tissue of mice in the treatment group by regulating the PPAR- 纬 -NF- 魏 p65 signal pathway in perivascular adipose tissue. Regulate the function of perivascular adipose tissue and inflammation, thus exerting the effect of anti-atherosclerosis.
【作者单位】：福建医科大学附属第一医院心脏外科;
【分类号】：R541.4

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