MicroRNA-499在新发心力衰竭患者治疗前后表达水平变化的研究

发布时间：2018-03-20 19:42

本文选题：新发心力衰竭　切入点：microRNA-499　出处：《宁夏医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的通过研究血清microRNA-499(miR-499)在新发心力衰竭(New Onset Heart Failure)患者治疗前后的表达变化,及其与氨基末端脑钠肽前体(N terminal B type natriuretic peptide,NT-proBNP)、左心室射血分数(left ventricular ejection fraction,LVEF)的相关性,评价miR-499在心力衰竭早期诊断中的价值,试图寻找一种早期诊断心衰的新的生物标志物。方法研究对象:2016年1月至2016年12月在上海市浦东新区公利医院心血管内科和急诊科收治并明确诊断为第一次发生心力衰竭的患者共30例(心衰组),按NYHA(美国纽约心脏病学会)标准对心功能进行分级。选择同年龄段在浦东新区公利医院体检中心的健康体检人员30例作为对照组。采用荧光定量RT-PCR的方法,检测心衰组所有个体治疗前后和健康对照组所有个体外周血miR-499的表达水平,比较心衰组和对照组人群血清中miR-499表达水平的差异,比较心衰患者治疗前后miR-499表达水平的变化,并分析miR-499与NT-proBNP、LVEF之间相关性。结果心力衰竭(heart failure,HF)组患者血清miR-499水平显著高于健康对照组(P0.05),且治疗后其表达水平下降;HF患者血清miR-499水平与NT-proBNP呈正相关,与LVEF呈负相关。另外,ROC曲线(受试者工作特征曲线)分析显示,对于HF的诊断miR-499具有特异性和敏感性,但并不优于NT-proBNP。结论初发心衰患者血清miR-499表达升高,且与患者心功能等级存在正相关。血清miR-499表达水平与NT-proBNP、LVEF具有较好的相关性。经抗心衰治疗后血清mi R-499水平显著下降。提示miR-499表达水平可能是一种早期诊断心力衰竭、评估患者病情严重程度的新的生物标志物。
[Abstract]:Objective to study the changes of serum microRNA-499miR-499 in patients with new Onset Heart before and after treatment, and its correlation with N-terminal brain natriuretic peptide precursor N terminal B type natriuretic peptide NT-proBNPN, left ventricular ejection fraction (LVEF) and left ventricular ejection fractionation (LVEF). To evaluate the value of miR-499 in early diagnosis of heart failure. Objective: to seek a new biomarker for early diagnosis of heart failure. Methods: from January 2016 to December 2016, the patients were admitted to Department of Cardiovascular Medicine and Emergency Department of Gongli Hospital, Pudong New District, Shanghai, and diagnosed for the first time. A total of 30 patients with heart failure (heart failure group) were classified according to NYHA (New York Heart Association) standard. 30 healthy persons of the same age at Pudong New area Gongli Hospital physical examination Center were selected as control group. The method of fluorescence quantitative RT-PCR was used. To detect the expression of miR-499 in peripheral blood of all individuals in heart failure group and healthy control group before and after treatment, to compare the difference of miR-499 expression in serum between heart failure group and control group, and to compare the changes of miR-499 expression level before and after treatment in heart failure patients. Results the level of serum miR-499 in patients with heart failure was significantly higher than that in healthy controls (P 0.05), and the level of serum miR-499 in patients with HF was positively correlated with that of patients with HF after treatment. In addition, the analysis of ROC curve showed that the diagnosis of miR-499 in HF was specific and sensitive, but not superior to that of NT-proBNP.Conclusion the expression of miR-499 in serum of patients with primary heart failure is higher than that of NT-proBNP.Conclusion the expression of miR-499 in patients with HF is higher than that of NT-proBNPs. There was a positive correlation between the expression of serum miR-499 and NT-proBNPP LVEF. The level of serum miR-499 decreased significantly after anti-heart failure treatment. It suggested that the expression of miR-499 might be an early diagnosis of heart failure. A new biomarker for assessing the severity of a patient's condition.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R541.6

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