黄芪多糖联合人脐带间充质干细胞改善糖尿病大鼠心肌病变的机制

发布时间：2018-03-24 21:33

本文选题：黄芪多糖　切入点：人脐带间充质干细胞　出处：《中国老年学杂志》2017年07期

【摘要】：目的从miRNAs角度探讨黄芪多糖(APS)联合人脐带间充质干细胞(HUCMSCs)改善糖尿病(DM)大鼠心肌病变的机制。方法取正常组(Con)、模型组(DM)及APS联合HUCMSCs治疗组(C)大鼠心肌组织进行miRNAs基因芯片检测,以Target Scan,miRanda,PicTar软件以及miRSystem网站(NTU,Taipei,Taiwan)对差异表达的miRNAs的靶基因及其耦联的信号通路进行预测及分析,并采用real-time qPCR验证芯片结果。结果 DM组与Con组以及C组均存在显著差异的miRNAs有8个,其中miR-34a-5p差异最明显,其靶基因有220个,可能耦联的信号通路主要有:Notch signaling pathway,p53 signaling pathway,MAPK signaling pathway,Cell cycle,Arrhythmogenic right ventricular cardiomyopathy(ARVC),且real-time qPCR检测miR-34a-5p的相对表达量与芯片结果一致。结论 APS联合HUCMSCs对DM大鼠心肌病变的改善作用可能是通过调控miR-34a-5p的表达实现的。
[Abstract]:Objective to investigate the mechanism of Astragalus Polysaccharide (APS) combined with human umbilical cord mesenchymal stem cells (HUCMSCs) in improving cardiomyopathy in rats with diabetes mellitus (DM). The target genes of differentially expressed miRNAs and their coupled signal pathways were predicted and analyzed by Target ScanmiRandy PicTar software and miRSystem website Taipei Taiwan. The results were verified by real-time qPCR. Results there were 8 miRNAs with significant differences between DM group and Con group and C group. Among them, the difference of miR-34a-5p was the most obvious, with 220 target genes. It is possible that the main signal pathways of the coupling are: Notch signaling pathway-p53 signaling pathway-MAPK signaling pathway mapk signaling cycle-Arrhymogenic right ventricular cardiopathy, and the relative expression of miR-34a-5p detected by real-time qPCR is consistent with the results of microarray. Conclusion the effect of APS combined with HUCMSCs on the improvement of cardiomyopathy in diabetic rats may be through regulation. The expression of miR-34a-5p is realized.
【作者单位】：吉林大学药学院;
【基金】：国家自然科学基金项目(81660531) 吉林省科技厅基础处自然科学基金项目(20150101228JC)
【分类号】：R587.2;R542.2

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