长期高盐所致高血压大鼠主动脉血管平滑肌细胞大电导钙激活钾通道电流的变化

发布时间：2018-03-27 16:41

本文选题：高盐饮食　切入点：高血压　出处：《中国动脉硬化杂志》2017年12期

【摘要】：目的观察高盐饮食后大鼠血压水平及主动脉血管平滑肌细胞(VSMC)大电导钙激活钾通道(BKCa)电流的变化。方法 3周龄雄性Wister大鼠随机分为2组:对照组和模型组,每组24只大鼠;对照组予以正常(含0.5%Na Cl)饮食,模型组予以高盐(含4%Na Cl)饮食。采用尾动脉测压法测量大鼠血压,全细胞膜片钳技术记录大鼠主动脉VSMC膜BKCa电流。结果模型组从第8周开始血压明显升高,随着高盐饮食时间延长,血压逐渐增加;在第10、12、14、16周时血压较对照组均显著升高(P0.01)。模型组大鼠主动脉VSMC膜BKCa电流和电流密度随高盐饮食时间延长而显著增大;在第8、12、16周时,BKCa峰电流密度较对照组明显升高,差异有统计学意义(P0.05)。结论高盐饮食可导致大鼠血压升高;大鼠BKCa电流密度随着高盐饮食所致血压的升高而逐渐增加。
[Abstract]:Objective to observe the changes of blood pressure and calcium activated potassium channel (BK Ca) current in aorta vascular smooth muscle cells (VSMC) after high salt diet. Methods male Wister rats aged 3 weeks were randomly divided into two groups: control group and model group with 24 rats in each group. The control group was fed with normal diet (including 0.5%Na Cl), the model group was fed with high salt diet (including 4%Na Cl), and the blood pressure of rats was measured by caudal artery manometry. Results the blood pressure of the model group increased significantly from the 8th week, and the blood pressure increased gradually with the prolongation of the time of high salt diet. At the 16th week, the blood pressure of the model group was significantly higher than that of the control group (P 0.01). The BKCa current and current density of the aortic VSMC membrane in the model group increased significantly with the prolongation of the time of high salt diet, and the peak current density of BK Ca in the rat aorta of the model group was significantly higher than that of the control group at the 8th week of 1216 weeks. Conclusion High salt diet can increase the blood pressure of rats, and the BKCa current density of rats increases with the increase of blood pressure induced by high salt diet.
【作者单位】：江西省人民医院江西省心血管病研究所;南昌大学江西医学院;江西省人民医院心内一科;
【基金】：国家自然科学基金资助项目(81260031) 江西省卫生计生委科技计划项目(20155056;20161002) 江西省科技厅科研院所基础设施配套项目(20142BBA13042)
【分类号】：R544.1

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