胆固醇对血管平滑肌细胞增殖及迁移的作用

发布时间：2018-03-31 06:02

本文选题：胆固醇　切入点：血管平滑肌细胞　出处：《中国动脉硬化杂志》2017年06期

【摘要】：目的探讨胆固醇对血管平滑肌细胞增殖及迁移的作用。方法体外培养人血管平滑肌细胞株(VSMC),以12.5 mg/L、25.0 mg/L、50.0 mg/L不同浓度胆固醇(CH)分别作用细胞24 h、48 h、72 h后,MTT法检测VSMC的增殖情况;流式细胞术检测细胞进入S期的数量;EDU试剂盒检测CH作用后细胞的DNA合成;划痕实验检测VSMC迁移情况;Real-time PCR检测细胞中miR145的表达水平。结果 12.5 mg/L、25.0 mg/L、50.0 mg/L CH作用VSMC后可增加细胞活力,促进细胞增殖,与对照组比较,进入S期的VSMC细胞数量明显增多,细胞迁移增加(P0.05);与对照组比较,25.0 mg/L、50.0 mg/L CH可诱导VSMC DNA合成增加,并且明显降低miR-145的表达水平(P0.05)。结论 CH可诱导VSMC进入S期细胞增多,促进细胞增殖,并且增加细胞的DNA合成及细胞迁移,其机制可能与抑制miR-145的表达有关。
[Abstract]:Objective to investigate the effect of cholesterol on proliferation and migration of vascular smooth muscle cells (VSMCs). Methods cultured human vascular smooth muscle cells (VSMCs) in vitro, the proliferation of VSMC was detected by MTT assay with 12.5 mg / L ~ 25.0 mg / L ~ (25. 0) mg / L ~ (50) mg/L for 24 h or 48 h / h for 72 h. Flow cytometry was used to detect the number of cells entering S phase. EDU kit was used to detect the DNA synthesis after Ch treatment. VSMC migration was detected by scratch test and real-time PCR was used to detect the expression of miR145. Results 12.5 mg / L ~ (25. 0) mg / L ~ (50) mg/L Ch could increase cell viability and promote cell proliferation after treatment with VSMC. Compared with the control group, the number of VSMC cells entering S phase was significantly increased. Compared with the control group, 50.0 mg/L Ch could induce the increase of VSMC DNA synthesis and decrease the expression level of miR-145. Conclusion Ch can induce the increase of VSMC into S phase cells and promote cell proliferation. The mechanism of increasing DNA synthesis and cell migration may be related to the inhibition of miR-145 expression.
【作者单位】：遵义医学院生物化学与分子生物学教研室;遵义医学院第一附属医院心内科;
【基金】：贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2015-152) 遵义医学院2012招标课题(F-613)
【分类号】：R54

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