NF-κB水平在大鼠血液高凝态下的变化

发布时间：2018-04-02 01:08

本文选题：高凝态　切入点：NF-κB　出处：《南京医科大学学报(自然科学版)》2017年08期

【摘要】：目的 :观察核因子κB(NF-κB)水平在大鼠血液高凝态下的变化,为血栓疾病的防治探索新靶点。方法 :大鼠80只随机分为模型组与生理盐水组,模型组以预实验确定的最适剂量盐酸肾上腺素造模,于12、18、24、48、96 h各组随机取8只大鼠颈动脉取血测凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、全血黏度,并采用酶联免疫吸附法测NF-κB水平。结果:0.7 mg/kg肾上腺素造模组模型复制成功率高,大鼠死亡率低,为最佳造模剂量。生理盐水组PT、APTT、全血黏度及NF-κB水平在各时间点无统计学差异;模型组PT、APTT较生理盐水组缩短(P0.05),但随时间缓慢延长,全血黏度及NF-κB水平较生理盐水组增大(P0.05),但随时间缓慢降低,且在12、18 h时PT、APTT同生理盐水组比较有显著差异(P0.05),24 h内全血黏度较生理盐水组显著增大(P0.05),48 h内NF-κB水平较生理盐水组显著升高(P0.05)。结论:高凝态大鼠血液中NF-κB水平显著升高,并随着高凝态的消退而降低,说明大鼠血液高凝态的发生、发展可能与NF-κB信号通路表达上调有关。
[Abstract]:Objective: to observe the changes of nuclear factor 魏 B (NF- 魏 B) NF- 魏 B level in blood hypercoagulable state of rats, and to explore new targets for the prevention and treatment of thrombotic diseases. Methods: 80 rats were randomly divided into model group and saline group. In the model group, the optimal dose of epinephrine hydrochloride was determined by pre-experiment. In each group, 8 rats in each group were randomly selected for measuring prothrombin time (PTT), activated partial thromboplastin time (APTTT) and whole blood viscosity. The level of NF- 魏 B was measured by enzyme-linked immunosorbent assay (Elisa). Results there was no significant difference in whole blood viscosity and NF- 魏 B level between normal saline group and control group. Compared with normal saline group, APTT in model group was shorter than that in saline group (P 0.05), but the whole blood viscosity and NF- 魏 B level increased as compared with normal saline group, but decreased slowly with time. The whole blood viscosity of PTT APTT was significantly higher than that of saline group in 24 h than that in saline group. Conclusion: the level of NF- 魏 B in blood of hypercoagulable rats was significantly higher than that of normal saline group in 48 h, and the level of NF- 魏 B in hypercoagulable rats was significantly higher than that in normal saline group within 48 h, conclusion: the blood NF- 魏 B level in hypercoagulable rats was significantly higher than that in saline group. With the decline of hypercoagulability, it is suggested that the development of hypercoagulability in rat blood may be related to the up-regulation of NF- 魏 B signaling pathway.
【作者单位】：无锡卫生高等职业技术学校护理系;中国药科大学药学院药理系;
【基金】：无锡市医院管理中心科研项目(YGZXM1533)
【分类号】：R54

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