阿司匹林对不稳定型心绞痛患者血浆中5种miRNA水平的影响

发布时间：2018-04-11 22:41

本文选题：阿司匹林 + 冠状动脉疾病　；参考：《重庆医学》2017年02期

【摘要】：目的探讨口服阿司匹林对不稳定心绞痛(UAP)患者血浆miR-21、miR-24、miR-126、miR-146、miR-223水平的影响。方法以未服用过阿司匹林的15例冠状动脉病变阳性的UAP患者及10例冠状动脉病变阴性的UAP患者作为研究对象,予以阿司匹林肠溶片0.1g,每日1次口服,实时定量反转录聚合酶链反应(RT-qPCR)检测miRNA浓度,比较服药前及服药1周后外周血浆中这5种miRNA的差异。同时选取50例UAP患者(包括冠状动脉病变阴性及阳性者),根据其近期是否服用阿司匹林情况分为阿司匹林组及对照组,比较不同组间血浆中5种miRNA水平。结果仅冠状动脉病变阳性的UAP患者服用阿司匹林后血浆中的miR-223水平升高有统计学意义(P0.05),而其他几种miRNA及冠状动脉病变阴性者血浆中5种miRNA水平变化均无统计学意义(P0.05)。结论阿司匹林可升高冠状动脉病变阳性的UAP患者血浆中miR-223的水平但不影响冠状动脉病变阴性者血浆miR-223水平。
[Abstract]:Objective to investigate the effect of oral aspirin on the plasma miR-21 miR-24 miR-126 miR-146 miR-146 miR-223 levels in patients with unstable angina pectoris (UAP).Methods Fifteen UAP patients with positive coronary artery disease and 10 UAP patients with negative coronary artery disease were treated with aspirin enteric-coated tablets (0.1g) once a day.Real-time quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) was used to detect the concentration of miRNA and to compare the five miRNA levels in peripheral plasma before and 1 week after administration.At the same time, 50 patients with UAP (including patients with negative and positive coronary artery lesions) were divided into aspirin group and control group according to whether they took aspirin recently. The plasma miRNA levels were compared among different groups.Results there was a significant increase in plasma miR-223 level in patients with positive coronary artery disease (UAP) only after taking aspirin, but there was no significant change in other miRNA and 5 miRNA levels in patients with negative coronary artery disease (P 0.05).Conclusion Aspirin can increase the level of plasma miR-223 in patients with positive coronary artery disease (UAP) but not affect the level of plasma miR-223 in patients with negative coronary artery disease.
【作者单位】：三峡大学医学院;三峡大学人民医院心内科;
【基金】：湖北省教育厅自然科学研究项目(Q20121306)
【分类号】：R541.4

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