新西兰兔再生障碍性贫血模型的建立

发布时间：2018-04-15 12:43

本文选题：再生障碍性贫血 + 苯　；参考：《南方医科大学学报》2017年12期

【摘要】：目的正交设计筛选苯和环磷酰胺联合使用建立新西兰兔再生障碍性贫血模型的适宜剂量。方法新西兰兔,采用正交试验设计,应用L9(34)正交表对苯的剂量(A)、环磷酰胺的剂量(B)、苯的注射次数(C)、环磷酰胺的注射次数(D)四个因素及它们各自不同的3个水平在建立新西兰兔再生障碍性贫血模型,并最终从9个实验组中优选出建模的较优方案。给药方法为先背部皮下注射苯,隔日1次,再耳缘静脉注射环磷酰胺,每天注射,均按照规定次数注射。每6 d检测1次血常规,于建模前、建模后36d取小段股骨进行骨髓组织学检查,观察变化。结果将9组白细胞、红细胞、血小板计数进行对比分析,4~9组新西兰兔再障模型建立成功,第7、8、9组与其他组日均下降速率之差有统计学意义(P0.05),其中第7组骨髓切片显示骨髓增生低下,造血组织减少,巨核细胞减少或消失,脂肪细胞增多。随访发现,第7组骨髓抑制一直存在,与该病临床特点相符合。结论第7组采用苯1.5 m L/kg,8次/d,环磷酰胺10 mg/kg,4次/d,这一组合剂量建立的新西兰兔AA模型建模周期短、且模型稳定,是一种可广泛应用于动物实验的建模方法。
[Abstract]:Objective to study the optimal dose of benzene and cyclophosphamide in aplastic anemia model in New Zealand rabbits.Methods New Zealand rabbits were designed by orthogonal test.In order to establish aplastic anemia model of New Zealand rabbits, four factors were used to establish aplastic anemia model in New Zealand rabbits, which were the dose of benzene, the dose of cyclophosphamide, the times of injection of benzene and the times of injection of cyclophosphamide, respectively.Finally, the optimal scheme of modeling was selected from 9 experimental groups.First, benzene was injected subcutaneously into the back, once every other day, and then cyclophosphamide was injected into the ear vein every day.Blood routine was detected once every 6 days, and bone marrow histological examination was performed 36 days after modeling.Results the white blood cell, red blood cell and platelet count in 9 groups were compared and analyzed. The model of aplastic anemia in 9 groups of New Zealand rabbits was established successfully.There was significant difference in the daily average rate of decline between group 7 and other groups (P 0.05). Bone marrow sections in group 7 showed low bone marrow proliferation, decreased hematopoietic tissue, decreased or disappeared megakaryocyte, and increased adipocytes.Follow-up showed that bone marrow suppression existed all the time in group 7, which was consistent with the clinical features of the disease.Conclusion the AA model of New Zealand rabbits established with benzene 1.5 mL / kg / kg 8 times / d and cyclophosphamide 10 mg / kg / d 4 times / d in the seventh group is a stable and stable model. It can be widely used in animal experiments.
【作者单位】：重庆医科大学生物医学工程学院//省部共建国家重点实验室培育基地-重庆市超声医学工程重点实验室//重庆市微无创医学协同创新中心;重庆医科大学附属第二医院;
【基金】：重庆市前沿与应用基础研究计划一般项目(cstc2016jcyj A0599)
【分类号】：R-332;R556.5

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