二氢杨梅素抑制棕榈酸诱导的血管内皮细胞焦亡

发布时间：2018-04-20 01:37

本文选题：动脉粥样硬化 + 二氢杨梅素　；参考：《第三军医大学学报》2017年05期

【摘要】：目的观察二氢杨梅素对棕榈酸刺激诱导的血管内皮细胞焦亡的影响。方法不同浓度棕榈酸处理人血管内皮细胞株EA.hy926 24 h,或以二氢杨梅素(0.1、0.5、1.0μmol/L)预处理12 h再用棕榈酸(200μmol/L)处理24 h后,CCK-8检测细胞活力,Cyto Tox 96检测细胞培养上清液乳酸脱氢酶水平,荧光显微镜检测细胞PI染色情况,caspase-1活性检测试剂盒检测caspase-1活性,Western blot法检测细胞caspase-1,IL-1β及ICAM-1蛋白表达。结果棕榈酸处理显著降低内皮细胞活力,同时显著增加乳酸脱氢酶释放水平、PI染色、caspase-1活性以及caspase-1、IL-1β、ICAM-1蛋白表达(P0.05)。二氢杨梅素预处理后,与棕榈酸单独处理组相比,细胞活力明显增加,乳酸脱氢酶释放水平及caspase-1活性显著降低(P0.05),同时caspase-1,IL-1β及ICAM-1蛋白表达水平也明显降低(P0.05)。结论棕榈酸可诱导内皮细胞发生焦亡,而二氢杨梅素可抑制棕榈酸诱导的血管内皮细胞焦亡作用。
[Abstract]:Objective to observe the effect of dihydromyricetin on palmitic acid-induced pyrolysis of vascular endothelial cells. Methods Human vascular endothelial cell line EA.hy926 was treated with palmitic acid at different concentrations for 24 h, or was pretreated with dihydromyricetin 0.51 (0.5 渭 mol 路L ~ (-1)) for 12 h and then treated with 200 渭 mol / L palmitic acid for 24 h. After 24 h treatment, the activity of the cells was detected by Cyto Tox 96, and the level of lactate dehydrogenase in supernatant of cell culture was measured. The expression of caspase-1 IL-1 尾 and ICAM-1 protein was detected by Western blot assay. Results palmitic acid treatment significantly decreased the activity of endothelial cells, significantly increased the level of lactate dehydrogenase release and the activity of caspase-1 by Pi staining. After pretreatment with dihydromyricetin, compared with palmitic acid alone, the cell viability was significantly increased, the level of lactate dehydrogenase release and caspase-1 activity decreased significantly, and the expression of caspase-1, IL-1 尾 and ICAM-1 protein decreased significantly. Conclusion palmitic acid can induce pyrolysis of endothelial cells, while dihydromyricetin can inhibit palmitic acid-induced apoptosis of vascular endothelial cells.
【作者单位】：第三军医大学军事预防医学院营养与食品卫生学教研室重庆市营养与食品安全重点实验室重庆市医学营养研究中心;
【分类号】：R54

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