贝那普利和氨氯地平对自发性高血压大鼠酪酪肽表达的影响

发布时间：2018-04-21 22:19

本文选题：自发性高血压大鼠 + 酪酪肽　；参考：《中国老年学杂志》2017年19期

【摘要】：目的探讨贝那普利和氨氯地平对自发性高血压大鼠(SHR)酪酪肽(PYY)表达的影响。方法 14周龄雄性SHR 45只随机分为模型组(n=15)、贝那普利组(盐酸贝那普利灌服0.90 mg·kg~(-1)·d~(-1),n=15)、氨氯地平组(苯磺酸氨氯地平灌服0.45 mg·kg~(-1)·d~(-1),n=15),以同源雄性WKY大鼠作为正常对照(n=15),模型组及WKY组每日灌服同体积的蒸馏水,干预8 w后,应用酶联免疫法检测血清中PYY的含量,采用免疫组化法和RT-PCR法分别检测结肠中PYY及其mRNA的表达。结果干预8 w后,模型组血清中PYY及其mRNA表达量显著升高(P0.05),与模型组比较,各给药组血清中PYY及其mRNA表达无显著性差异(P0.05);与WKY组比较,模型组结肠组织中PYY及其mRNA表达显著升高(P0.05),与模型组比较,贝那普利组与氨氯地平组结肠组织中PYY及其mRNA表达降低(P0.05),与贝那普利组比较,氨氯地平组结肠组织中PYY及其mRNA表达显著降低(P0.05)。结论 SHR大鼠结肠组织中PYY调节失衡,贝那普利、氨氯地平的降压作用很可能还通过调节PYY的表达而达到降压目的。
[Abstract]:Objective to investigate the effects of benazepril and amlodipine on the expression of tyrosine tyrosine in spontaneously hypertensive rats. Methods Forty-five 14-week-old male SHR rats were randomly divided into three groups: model group (n = 15), benazepril group (n = 0.90 mg 路kg ~ (-1)) and amlodipine group (n = 0.45 mg 路kg ~ (-1)) d ~ (-1) respectively. The homologous male WKY rats were used as control group. And WKY group were given distilled water of the same volume daily. After 8 weeks of intervention, the levels of PYY in serum were detected by enzyme-linked immunosorbent assay (Elisa), and the expression of PYY and mRNA in colon were detected by immunohistochemical method and RT-PCR method, respectively. Results after 8 weeks of intervention, the expression of PYY and its mRNA in serum of the model group was significantly higher than that of the model group. There was no significant difference in the expression of PYY and mRNA in the serum of the model group compared with the model group, and the expression of PYY and mRNA in the serum of the model group was significantly higher than that in the WKY group. Compared with the model group, the expression of PYY and mRNA in the colonic tissue of benazepril group and amlodipine group decreased significantly (P 0.05), and that of benazepril group and amlodipine group was lower than that of benazepril group, and that of benazepril group was significantly higher than that of benazepril group. The expression of PYY and mRNA in colon tissue of amlodipine group was significantly lower than that of amlodipine group. Conclusion the regulation of PYY in colon tissue of SHR rats is out of balance. The antihypertensive effect of benazepril and amlodipine may also be achieved by regulating the expression of PYY.
【作者单位】：甘肃中医药大学;
【基金】：国家自然科学基金资助项目(81160477) 甘肃省自然科学研究基金计划(1107RJZA220) 甘肃省高等学校基本科研业务费资助项目(2010-5)
【分类号】：R544.1

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