HMGB1预处理骨髓间充质干细胞移植治疗心肌梗死大鼠的实验研究

发布时间：2018-04-22 09:33

本文选题：高迁移率族蛋白- + 预处理　；参考：《临床心血管病杂志》2017年07期

【摘要】：目的:研究高迁移率族蛋白-1(1HMGB1)预处理的骨髓间充质干细胞(BMSCs)移植治疗大鼠心肌梗死的有效性及其机制。方法:建立急性心肌梗死(AMI)大鼠模型,体外传代培养大鼠BMSCs,以25.0μg/L HMGB1预处理细胞后48h移植到大鼠心脏梗死区域。实验分为假手术组、模型对照组、BMSCs组、HMGB1+BMSCs组。心电图检测确定心肌梗死模型的建立。细胞移植28d后进行以下检测:HE、Masson染色检测梗死心脏形态学变化;TTC染色测定心肌梗死面积;超声心动图测定心脏功能;免疫组织化学染色检测心肌梗死交界区血小板内皮细胞黏附分子-1(CD31)的表达;Western-blot检测梗死心肌组织血管内皮生长因子(VEGF)的表达。结果:心电图结果表明大鼠心肌梗死模型成功建立。与模型对照组相比,细胞移植组能有效减轻大鼠心肌纤维化程度及梗死面积,以HMGB1+BMSCs组更为明显(P0.05)。单纯BMSCs以及HMGB1预处理BMSCs移植均能增加心肌梗死区CD31、VEGF的表达,有效改善梗死心脏功能,HMGB1预处理作用更显著(P0.05)。结论:HMGB1预处理BMSCs较单纯的BMSCs移植更能有效减轻心肌梗死程度,改善心脏功能,并且该效应可能与HMGB1促进BMSCs的旁分泌有关。
[Abstract]:Aim: to study the efficacy and mechanism of bone marrow mesenchymal stem cell transplantation (BMSCs) pretreated with high mobility group protein-1HMGB1 in the treatment of myocardial infarction in rats. Methods: acute myocardial infarction (AMI) rat model was established. BMSCs were cultured in vitro. The cells were pretreated with 25.0 渭 g / L HMGB1 for 48 hours. The experiment was divided into sham operation group and model control group. The establishment of myocardial infarction model was determined by electrocardiogram. After 28 days of cell transplantation, the following methods were used to detect the morphological changes of the infarcted heart and the area of myocardial infarction by TTC staining and echocardiography, respectively. Immunohistochemical staining was used to detect the expression of platelet endothelial cell adhesion molecule-1 (CD31) and Western-blot was used to detect the expression of vascular endothelial growth factor (VEGF) in myocardial infarction. Results: electrocardiogram showed that myocardial infarction model was successfully established in rats. Compared with the model control group, the degree of myocardial fibrosis and the infarct size of the model group were effectively alleviated in the cell transplantation group, especially in the HMGB1 BMSCs group. Both BMSCs and HMGB1 preconditioning BMSCs transplantation could increase the expression of CD31 BMSCs in myocardial infarction area, and improve the myocardial function of infarcted heart. HMGB1 preconditioning could significantly improve the expression of CD31 BMSCs in myocardial infarction area (P0. 05). Conclusion compared with BMSCs transplantation, BMSCs pretreated with BMSCs at 1: HMGB1 is more effective in reducing myocardial infarction and improving cardiac function, and this effect may be related to the promotion of paracrine of BMSCs by HMGB1.
【作者单位】：遵义医学院附属医院心内科;遵义医学院生物化学与分子生物学教研室;
【基金】：贵州省科技计划课题(No:黔科合SY字(2012)3109号) 遵义市科技计划课题(No:遵市科合社字(2014)82号)
【分类号】：R542.22

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