血清miR-499和miR-652用于急性冠脉综合征早期诊断的临床试验

发布时间：2018-04-22 16:24

本文选题：微小RNA + 急性冠脉综合征　；参考：《上海交通大学学报(医学版)》2017年09期

【摘要】：目的·评估血清mi RNA用于急性冠脉综合征(ACS)早期诊断的潜在价值。方法·急诊入院的28例疑似ACS患者,在3 h内采集血液样本;根据ACS诊疗指南,最终将患者分为ACS组(18例)和非ACS组(10例)。采用q RT-PCR技术检测心肌特异性mi R-499以及与心肌损伤密切相关的mi R-652的含量,同时监测肌钙蛋白I(c Tn I)变化,分析mi RNA和c Tn I之间的相关性,并采用95%界值建立医学参考值范围。结果·研究对象急诊入院3 h内,ACS患者的血清中已检测到mi RNA的升高;并且ACS组患者血清mi R-499的表达水平为非ACS组的9.2倍(P=0.009)。mi R-499(r=0.595,P=0.001)和mi R-652(r=0.579,P=0.001)均与c Tn I呈显著正相关;mi R-499和mi R-652的ROC曲线下面积(AUC)分别为0.786和0.583;mi R-499的敏感度与特异度分别为72.22%和80.00%,mi R-652则为72.22%和60.00%;mi R-499和mi R-652医学参考值范围分别为0.001~2.723和0.122~9.660。结论·血清中的心肌特异性mi R-499有望成为ACS早期诊断的生物标志物。
[Abstract]:Objective to evaluate the potential value of serum mi RNA in the early diagnosis of acute coronary syndrome (ACS). Methods Blood samples were collected from 28 suspected ACS patients in emergency department within 3 hours, and were divided into ACS group (n = 18) and non- group (n = 10) according to the guidelines of ACS diagnosis and treatment. The contents of myocardial specific mi R-499 and miR-652, which were closely related to myocardial injury, were detected by Q RT-PCR technique, and the changes of troponin I C Tn I were monitored, and the correlation between mi RNA and c TN I was analyzed. The range of medical reference value was established by 95% boundary value. Results the increase of mi RNA was detected in the serum of patients with ACS within 3 hours after admission to hospital. And the positive correlation between the expression levels of serum mi R-499 and c TNI in the ACS group was 9.2-fold Pn.0.595P0. 001) and in the non- group (0.786 and 0.583% respectively). The sensitivity and specificity of the area under the ROC curve were 72.22% and 0.583%, respectively, for the ROC curve of the patients with ACS and the ROC curve of mi R-652 were 0.786 and 0.583%, respectively.The sensitivity and specificity were 72.22% and 72.22%, respectively, for the area under the ROC curve of the patients with ACS and the ROC curve of the MI R-652 were 0.786 and 0.583% respectively. The medical reference values of R- 652 and R- 652 were 72.22% and 60.00mm-R-499 and R-652, respectively, in the range of 0.001 / 2.723 and 0.122 / 9.660, respectively. Conclusion Myocardial specificity mi R 499 in serum may be a biomarker for early diagnosis of ACS.
【作者单位】：上海交通大学医学院附属新华医院麻醉与重症医学科;
【基金】：国家自然科学基金(81470390) 上海市教育委员会高峰高原学科建设计划(20152218) 上海申康临床三年行动计划(16CR3006A)~~
【分类号】：R541.4

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