MGP与主动脉瓣膜钙化的相关性研究

发布时间：2018-04-25 13:24

本文选题：基质Gla蛋白 + 主动脉瓣膜　；参考：《新乡医学院》2017年硕士论文

【摘要】：背景主动脉瓣膜钙化性疾病(Calcific Aortic Valve Disease,CAVD)的发病率随着老龄化的加剧逐年升高,具体机制不清楚。CAVD是调控机制复杂的瓣膜间质细胞成骨样分化过程。实验发现,基质Gla蛋白(Matrix Gla Protein MGP)与动物主动脉瓣膜钙化有关,是瓣膜钙化的一种抑制剂,在血清和瓣膜组织中均有表达。但关于MGP与人主动脉瓣膜的关系尚未见报道。目的检测MGP在人主动脉瓣膜钙化中的表达情况,反映MGP与人主动脉瓣膜钙化的关系。方法1.需行瓣膜置换的无病变的马方综合症及主动脉夹层患者的主动脉瓣膜10例做对照组;单纯主动脉瓣膜钙化需行主动脉置换患者的瓣膜做实验组,结合术前超声及术中探查情况分为中度钙化组10例,重度钙化组10例;2.术前30分钟分别取正常组及中、重度钙化组患者血标本;3.Elisa法、Western-Blot法测定对照组及实验组血清及瓣膜组织中MGP水平。结果主动脉瓣膜MGP的表达与对照组相比,中、重度钙化组主动脉瓣膜MGP表达水平降低(P0.05);血清MGP蛋白水平与对照组相比,中、重度钙化组的主动脉瓣膜MGP水平降低(P0.05)。结论本研究发现,随着钙化的加重,血清及瓣膜中MGP表达量逐渐降低,特别是重度钙化组中MGP表达量明显降低。说明MGP的表达量随瓣膜钙化程度的加重呈降低趋势。
[Abstract]:Background the incidence of aortic valve calcification disease Calcific Aortic Valve disease is increasing with the aggravation of aging, and the mechanism is not clear. CAVD is the process of osteoblast-like differentiation of valvular interstitial cells with complex regulation mechanism. It was found that matrix Gla Protein MGPs are associated with aortic valve calcification and are an inhibitor of valvular calcification, and are expressed in both serum and valve tissues. However, the relationship between MGP and human aortic valve has not been reported. Aim to detect the expression of MGP in human aortic valve calcification and to reflect the relationship between MGP and human aortic valve calcification. Method 1. The control group consisted of 10 patients with valvular valvular disease and aortic dissection, and 10 patients with aortic valve calcification were treated with aortic valve replacement as experimental group. Combined with preoperative ultrasound and intraoperative exploration, 10 cases were divided into moderate calcification group (n = 10) and severe calcification group (n = 10). Serum MGP levels in control group and experimental group were determined by Western-Blot 30 minutes before operation. Results compared with the control group, the expression of MGP in aortic valve was significantly decreased in moderate calcified group and serum MGP protein level was decreased in moderate calcified group compared with control group, while in severe calcified group, the MGP level of aortic valve was lower than that of control group. Conclusion with the increase of calcification, the expression of MGP in serum and valve decreased gradually, especially in the severe calcification group. The results showed that the expression of MGP decreased with the severity of calcification.
【学位授予单位】：新乡医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R542.52

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