微纤维蛋白1在瓣膜性心房颤动患者心房组织中的表达及其与心房纤维化的关系

发布时间：2018-05-01 08:17

本文选题：风湿性心脏瓣膜病 + 心房颤动　；参考：《吉林大学学报(医学版)》2017年02期

【摘要】：目的:观察微纤维蛋白1(FBN-1)在风湿性心脏瓣膜病并发心房颤动(AF)患者心房组织中的表达,探讨FBN-1与心房纤维化的关系。方法:选择因风湿性心脏瓣膜病住院并行瓣膜置换手术的患者84例,分为AF组39例和窦性心律(SR)组45例;收集整理患者的临床资料,并于手术中获取右心房组织(0.3~0.5mm3)。Masson染色观察2组患者右心房纤维化程度;Western blotting法测定各组患者心房组织中FBN-1蛋白的表达,相关分析探讨FBN-1与心房纤维化的关系。结果:2组患者在性别构成比、年龄、血压、血常规和生化指标等方面比较差异无统计学意义(P0.05)。AF组患者左和右心房直径明显大于SR组(P0.05);Masson染色,AF组患者心房组织存在明显纤维化,AF组患者胶原容积分数和胶原水平明显高于SR组(P0.05);AF组患者心房组织中FBN-1的蛋白表达水平明显高于SR组(P0.05);瓣膜性AF患者右心房组织FBN-1蛋白表达水平与胶原水平呈正相关关系(r=0.544,P=0.021)。结论:瓣膜性AF患者心房组织存在明显的心房纤维化,与FBN-1基因转录水平上调有关联。
[Abstract]:Aim: to investigate the expression of microfibrillar protein 1 (FBN-1) in atrial tissue of patients with rheumatic valvular heart disease complicated with atrial fibrillation (AF), and to investigate the relationship between FBN-1 and atrial fibrosis. Methods: 84 patients with rheumatic valvular disease were divided into AF group (n = 39) and sinus arrhythmia group (n = 45). The degree of right atrial fibrosis in two groups was observed by Western blotting method. The expression of FBN-1 protein was detected by Western blotting. The correlation analysis was carried out to explore the relationship between FBN-1 and atrial fibrosis. Results the sex composition ratio, age, blood pressure of two groups of patients, There was no significant difference in blood routine and biochemical indexes. The diameter of left and right atrium in AF group was significantly larger than that in SR group (P 0.05 Masson staining group). Collagen volume fraction and collagen water were found in AF group and AF group. The expression level of FBN-1 protein in atrial tissue of patients with AF was significantly higher than that of SR group (P 0.05), and the expression of FBN-1 protein in right atrium of patients with valvular AF was positively correlated with the level of collagen. Conclusion: atrial fibrosis in patients with valvular AF is associated with up-regulation of FBN-1 gene transcription.
【作者单位】：西北大学生命科学学院预防基因组医学研究所;第四军医大学附属西京医院心血管内科;西安交通大学医学部第一附属医院心血管内科;
【基金】：陕西省自然科学基金资助课题(S2017-ZRJJ-1848)
【分类号】：R541.75

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