基于毛细管电泳的地中海贫血分析系统研制

发布时间：2018-05-01 08:26

  本文选题：地中海贫血 + 毛细管电泳　； 参考：《深圳大学》2017年硕士论文

【摘要】：地中海贫血是一种高发于我国长江以南地区的遗传性溶血性疾病,通过检测血液中血红蛋白变异体的存在而确定地中海贫血病的高效毛细管电泳法是目前医用最常规的地贫筛查手段。但是目前国内市面上的医用毛细管电泳仪多为外国生产,而且体积庞大,价格昂贵。因此,本课题自主研发制造了一套基于毛细管电泳的地中海贫血分析系统,以填补国内毛细管电泳仪器开发领域的空白。首先,对高效毛细管电泳法进行原理性研究,并结合地中海贫血检测的相关特征,提出毛细管电泳系统的设计需求和设计方案。然后,根据系统设计方案完成系统机械结构、系统液路结构、系统光路结构、系统硬件电路结构和系统软件的设计。其中,系统机械结构和系统液路的设计满足毛细管电泳系统框架与密闭性的需求。系统光路设计满足地中海贫血样品中血红蛋白特异性波长检测的需求。系统硬件电路中:可编程供电电源电路满足系统各模块供电需求;可编程高压电源电路满足系统电泳电压0-12 kV连续可调需求;精密温控电路满足控制系统电泳温度37℃需求;运动控制电路满足系统自动化和毛细管nL级别进样需求;检测电路满足系统极微弱光检测需求。最后,使用KeilμVision5编写下位机驱动软件,使用Visual Studio 2015编写上位机界面软件,并对系统软硬件电路进行上下位机联合调试。在系统平台搭建完成后,进行系统关键模块,包括高压电源模块、温度控制模块和检测模块的测试。在测试合格后进行系统精密度和灵敏度的测试,并通过重复多次质控样品实验对系统电泳电压、进样量、进样浓度与光源强度等多个参数进行优化。在系统优化完成后进行地中海贫血血样上机实验,包括质控液毛细管电泳实验、α-地中海贫血毛细管电泳实验和β-地中海贫血毛细管电泳实验。实验结果表明,本课题设计的毛细管电泳系统在电泳电压9800 V、样品稀释6倍时灵敏度最高;系统精密度小于2%,满足地中海贫血血红蛋白检测需求;且本系统能够成功完成典型的α-地贫和β-地贫血样分析检查。
[Abstract]:Thalassemia is a highly prevalent hereditary hemolytic disease in the area south of the Yangtze River in China. High performance capillary electrophoresis (HPCE), which detects the presence of hemoglobin variants in blood, is the most common screening method for thalassemia. But at present, most of the medical capillary electrophoresis instruments on the domestic market are produced by foreign countries, and the volume is huge and the price is expensive. Therefore, a new analytical system of thalassemia based on capillary electrophoresis was developed to fill the blank in the field of capillary electrophoresis in China. Firstly, the principle of high performance capillary electrophoresis (HPCE) was studied, and the design requirements and scheme of capillary electrophoresis system were put forward according to the characteristics of thalassemia detection. Then, according to the system design scheme, the system mechanical structure, the system fluid structure, the system optical circuit structure, the system hardware circuit structure and the system software design are completed. Among them, the mechanical structure and liquid circuit design of the system meet the requirements of capillary electrophoresis system framework and sealing. The systematic optical circuit design meets the requirement of hemoglobin specific wavelength detection in thalassemia samples. In the hardware circuit of the system, the programmable power supply circuit can meet the demand of each module of the system, the programmable high voltage power supply circuit can meet the requirements of the system electrophoretic voltage 0-12 kV continuously adjustable, the precision temperature control circuit meets the requirement of the control system electrophoresis temperature 37 鈩，

本文编号：1828535