线粒体途径在免疫性骨髓衰竭性血小板凋亡中的作用机制研究

发布时间：2018-05-08 22:41

本文选题：线粒体 + 骨髓衰竭　；参考：《中国实验血液学杂志》2017年01期

【摘要】：目的:探讨线粒体途径在免疫性骨髓衰竭性血小板凋亡中的作用机制。方法:将30只C57BL/6小鼠随机分为3组:正常组、模型组和环孢素(CsA)组,每组10只。建立免疫性骨髓衰竭模型。造模成功后,给予正常组和模型组小鼠生理盐水灌胃,给予CsA组小鼠CsA灌胃。用自动血细胞分析仪检测各组小鼠血常规指标;采用流式细胞术测定线粒体膜电位(△ψm)、细胞色素C(Cyt C)、磷脂酰丝氨酸(PS)、Ca2+水平;采用Western blot法检测BAX、BAK、caspase-3、caspase-8、caspase-9的表达;在透射电子显微镜下观察骨髓血小板超微结构的变化。结果:与正常组比较,模型组血小板计数明显下降,△Ψm、caspase-3、caspase-8、caspase-9水平明显下降,Cyt C、PS、Ca~(2+)、BAX、BAK水平明显升高(P0.05)。与模型组比较,CsA组血小板计数明显升高,CsA对照组△Ψm、caspase-3、caspase-8、caspase-9水平明显升高,CsA对照组Cyt C、PS、Ca~(2+)、BAX、BAK水平明显下降(P0.05)。在电子显微镜下CsA组血小板结构损伤程度均较模型对照组有所减轻。结论:线粒体途径在免疫性骨髓衰竭性血小板减少中起重要作用。
[Abstract]:Objective: to investigate the mechanism of mitochondrial pathway in platelet apoptosis induced by immune bone marrow failure. Methods: thirty C57BL/6 mice were randomly divided into 3 groups: normal group, model group and cyclosporine CSA group with 10 in each group. To establish immune bone marrow failure model. After the model was successfully established, normal saline was given to normal group and model group, and CsA was given to CsA in CsA group. The levels of mitochondrial membrane potential (蠄 mtc), cytochrome C(Cyt, phosphatidyl serine (PSN) Ca 2, and the expression of BAXBAC caspase-3 and caspase-8 in each group were detected by automatic blood cell analyzer (ACS), flow cytometry (FCM), and the expression of caspase-8 caspase-9 in BAXBAK3 caspase-3 by flow cytometry (FCM). The ultrastructure of bone marrow platelets was observed under transmission electron microscope. Results: compared with the normal group, the platelet count in the model group was significantly decreased, and the level of caspase-8 and caspase-9 was significantly decreased in the model group. Compared with the model group, the platelet count in CSA group was significantly higher than that in CSA control group. The level of caspase-3, caspase-8 and caspase-9 in CSA control group was significantly higher than that in CSA control group, and the level of BAK in Cyt caspase-3, caspase-8 and caspase-9 in CSA control group was significantly lower than that in CSA control group (P 0.05). The degree of platelet structure damage in CsA group was less than that in model group under electron microscope. Conclusion: mitochondrial pathway plays an important role in immune bone marrow failure thrombocytopenia.
【作者单位】：上海市宝山区中西医结合医院血液科;
【基金】：上海市宝山区中西医结合医院科研项目(201601);上海市宝山区中西医结合医院国家自然基金培育专项项目(GZRPYJJ-201601) 宝山区卫生青年医学人才培养计划(bswsyq-2016-A11) 上海市卫生和计划生育委员会科研课题(201640144)
【分类号】：R551.3

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