原花青素B2保护血管内皮细胞延缓凋亡及机制研究

发布时间：2018-05-09 16:00

本文选题：原花青素B + 凋亡　；参考：《重庆医学》2017年13期

【摘要】：目的以H_2O_2诱导人内皮细胞凋亡为模型,观察原花青素B2(GSPB2)延缓人内皮细胞凋亡的保护作用及其机制。方法用0.5mmol H_2O_2预处理细胞,分别加入不同浓度的GSPB2(5.0、10.0、20.0μmol/L)保护细胞,TUNEL法检测细胞凋亡;Western blotting检测凋亡相关分子Caspase-3、Bax、Bcl-2以及影响凋亡的相关分子PI3K、p-Akt、Akt的蛋白表达;Transwell小室检测各组细胞迁移情况。结果 0.5mmol H_2O_2致人内皮细胞凋亡明显增加(P0.01),GSPB2能明显减轻H_2O_2所致的人内皮细胞凋亡(P0.05),10μmol/mL的GSPB2就能接近恢复到H_2O_2未处理的对照组水平。进一步研究发现:Caspase-3、bax蛋白表达上调,Bcl-2表达下调;PI3K、p-Akt、Akt表达上调。结论不同浓度的GSPB2对H_2O_2诱导的血管内皮细胞具有保护作用,而且表现为浓度依赖性和时间依赖性,其机制与相关分子PI3K、p-Akt、Akt有关。
[Abstract]:Objective to observe the protective effect of procyanidin B _ 2 (GSPB _ 2) on human endothelial cell apoptosis induced by H_2O_2 and its mechanism. Methods the cells were pretreated with 0.5mmol H_2O_2 and the cells were treated with different concentrations of GSPB _ 2 5.0 ~ 10.0 渭 mol 路L ~ (-1). Tunel method was used to detect apoptosis. Western blotting was used to detect the expression of apoptosis related molecule Caspase-3, Baxanine Bcl-2, and the protein expression of PI3KP- Akt, which was associated with apoptosis, to detect the migration of the cells in each group. Results the apoptosis of human endothelial cells induced by 0.5mmol H_2O_2 increased significantly (P 0.01). GSPB2 could significantly reduce the apoptosis of human endothelial cells induced by H_2O_2. The GSPB2 of 10 渭 mol/mL of P0.05 or 10 渭 mol/mL could return to the untreated control level of H_2O_2. Further studies showed that the expression of Bcl 2 was down-regulated by up-regulation of the protein expression of Caspase-3 and up-regulation of Akt. Conclusion different concentrations of GSPB2 have protective effects on H_2O_2 induced vascular endothelial cells in a concentration and time dependent manner, and the mechanism is related to the related molecule PI3KP- Akt.
【作者单位】：重庆市南川区人民医院心内科;第三军医大学野战外科研究所大坪医院心内科;
【分类号】：R54

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