成年大鼠心室肌细胞表达多种电压门控钠通道α亚型

发布时间：2018-05-13 00:26

本文选题：心室肌细胞 + 电压门控钠通道　；参考：《西安交通大学学报(医学版)》2017年06期

【摘要】：目的观察成年大鼠心室肌细胞上电压门控钠通道不同亚型的表达情况。方法采用酶解消化法分离成年大鼠心室肌细胞,利用免疫荧光染色结合激光共聚焦技术检测心室肌细胞钠通道α亚型Nav1.1、Nav1.2、Nav1.3、Nav1.5、Nav1.6、Nav1.7的表达及分布情况,采用全细胞膜片钳技术记录心室肌细胞钠电流。结果成年大鼠心室肌细胞除表达心脏型钠通道亚型Nav1.5之外,还表达神经型钠通道亚型Nav1.1、Nav1.6和Nav1.7,不表达Nav1.2和Nav1.3。Nav1.5分布于横小管周围,Nav1.1和Nav1.7亦呈点状分布于横小管区域。Nav1.6表达稍弱,沿心室肌细胞长轴纵向分布,闰盘处未见钠通道各α亚型的表达。膜片钳结果显示成年大鼠心室肌细胞表达快钠电流(INa,T)及晚钠电流(INa,L)。结论成年大鼠心室肌细胞表达心脏型钠通道亚型(Nav1.5)和多种神经型钠通道亚型(Nav1.1、Nav1.6和Nav1.7),这可能有助于维持心肌细胞的正常电生理活动。
[Abstract]:Objective to investigate the expression of voltage-gated sodium channels in adult rat ventricular myocytes. Methods Adult rat ventricular myocytes were isolated by enzymatic digestion. The expression and distribution of Na channel 伪 subtype Nav1.1 + Nav1.2 + Nav1.3Nv1.5Nv1.6Nv1.7 in ventricular myocytes were detected by immunofluorescence staining and confocal laser technique. The sodium currents in ventricular myocytes were recorded by whole cell patch clamp technique. Results in addition to the expression of cardiac sodium channel subtype Nav1.5, adult rat ventricular myocytes also expressed neuronal sodium channel subtype Nav1.1 Nav1.6 and Nav1.7, and did not express Nav1.2 and Nav1.3.Nav1.5 in the lateral tubules. The expression of Nav1.2 and Nav1.3.Nav1.5 was also slightly weaker in the transverse tubule region. Along the long axis of ventricular myocytes, no 伪 subtypes of sodium channel were found in intercalated disc. The patch-clamp results showed that rat ventricular myocytes expressed fast sodium current and late sodium current. Conclusion Adult rat ventricular myocytes express cardiac sodium channel subtype Nav1.5) and several neural sodium channel subtypes Nav1.1 Nav1.6 and Nav1.7, which may contribute to the maintenance of normal electrophysiological activities of cardiac myocytes.
【作者单位】：西安交通大学第一附属医院心血管内科陕西省分子心脏病学重点实验室环境与疾病相关教育部重点实验室;
【基金】：国家自然科学基金资助项目(No.30830051,30801216)~~
【分类号】：R54

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