载脂蛋白A5基因多态性与冠心病患者血脂及冠脉狭窄程度的相关性

发布时间：2018-05-19 12:22

本文选题：载脂蛋白A + 多态性　；参考：《中山大学学报(医学科学版)》2017年05期

【摘要】：【目的】探讨中国汉族人群载脂蛋白A5(APOA5)基因rs2075291和rs3135507多态性位点与冠心病(CHD)患者血脂水平及冠脉狭窄程度的关系。【方法】应用聚合酶链式反应-限制性片段长度多态性法(PCR-RFLP)对324例CHD患者和152例非CHD对照者进行rs2075291和rs3135507多态性分型并比较不同基因型之间血脂水平的差异。采用Gensini评分系统评价CHD患者的冠脉狭窄程度,并使用多因素线性回归法分析rs2075291和rs3135507多态性以及其他因素与Gensini得分之间的相关性。【结果】CHD组高血压患病率、甘油三酯(TG)、总胆固醇(TC)、载脂蛋白B100(APOB100)、脂蛋白a[Lp(a)]、TG/高密度脂蛋白胆固醇(HDL-C)、TC/HDL-C、低密度脂蛋白胆固醇(LDL-C)/HDL-C和APOB100/载脂蛋白AI(APOAI)水平高于对照组,HDL-C和APOAI水平低于对照组,差异均有统计学意义(P0.05)。rs2075291和rs3135507多态性等位基因及基因型分布频率在CHD组与对照组之间差异无统计学意义。在对照组中,rs2075291多态性GT基因型TG和TG/HDL-C水平高于GG基因型,HDL-C水平低于GG基因型,差异均有统计学意义(P0.05);在CHD组中,rs2075291多态性GT基因型TG/HDL-C和TC/HDL-C高于GG基因型,差异有统计学意义(P0.05)。在CHD组中,rs3135507位点A等位基因携带者APOB100水平低于GG基因型,差异有统计学意义(P0.05)。多因素回归分析结果显示,rs2075291和rs3135507多态性与Gensini得分的相关性无统计学意义(P0.05)。【结论】APOA5 rs2075291影响血脂水平,但与CHD发生发展无明显相关性,此研究结果需大样本多中心的病例-对照研究加以证实。
[Abstract]:[objective] to investigate the relationship between rs2075291 and rs3135507 polymorphism of apolipoprotein A5apOA5 gene and the level of blood lipid and the degree of coronary stenosis in patients with coronary heart disease (CHD). [methods] Polymerase chain reaction-restriction fragment length was used. The polymorphism of rs2075291 and rs3135507 were detected by PCR-RFLP in 324 CHD patients and 152 non-CHD controls, and the differences of blood lipid levels among different genotypes were compared. The degree of coronary artery stenosis in patients with CHD was evaluated by Gensini scoring system, and the correlation between rs2075291, rs3135507 polymorphism and other factors and Gensini score was analyzed by multivariate linear regression. [results] the prevalence of hypertension in CHD group was analyzed. The levels of TG / HDL-C, LDL-CU HDL-C and APOB100/ apolipoprotein AIAPOAI in the control group were higher than those in the control group, and the levels of HDL-C and APOAI in the control group were significantly higher than those in the control group, and the levels of HDL-C and APOAI in the control group were lower than those in the control group, and the levels of HDL-C / HDL-C were higher than those in the control group, and the levels of HDL-C and APOAI in the control group were significantly higher than those in the control group, and the levels of HDL-C / HDL-C were higher than those in the control group. There was no significant difference in alleles and genotypes between CHD group and control group. In the control group, the TG and TG/HDL-C levels of rs2075291 polymorphism GT genotype were higher than those of GG genotype and HDL-C level was lower than that of GG genotype, the difference was statistically significant (P 0.05), while the TG/HDL-C and TC/HDL-C levels of rs2075291 polymorphism GT genotype in CHD group were higher than those of GG genotype (P 0.05). In CHD group, the APOB100 level of allele A at Rs3135507 was lower than that of GG genotype, and the difference was statistically significant (P 0.05). Multivariate regression analysis showed that rs2075291 and rs3135507 polymorphism had no significant correlation with Gensini score. [conclusion] APOA5 rs2075291 affects blood lipid level, but has no significant correlation with the occurrence and development of CHD. This study needs to be confirmed by a large-sample multi-center case-control study.
【作者单位】：川北医学院附属医院心内科;雅安职业技术学院医学系;川北医学院药学院药物研究所;川北医学院临床医学系;川北医学院医学检验系;川北医学院基础医学院生化教研室;
【基金】：四川省教育厅重点项目(17ZA0172) 川北医学院科研创新团队项目(CBY13-TD02)
【分类号】：R541.4

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