线粒体DNA含量与急性冠脉综合征的关系

发布时间：2018-05-19 18:09

本文选题：mtDNA含量 + 急性冠脉综合征　；参考：《实用医学杂志》2017年20期

【摘要】：目的探讨外周血线粒体DNA(mitochondrial DNA,mtDNA)含量与急性冠脉综合征(acutecoronary syndrome,ACS)的关系。方法连续入选于承德医学院附属医院行冠状动脉造影的患者244例,根据造影结果将入选者分为ACS组(183例)和对照组(61例)。采用实时定量PCR的方法检测2组外周血mtDNA含量,ACS组根据病变血管支数分为单支病变组、双支病变组、三支病变组,Gensini评分定量评估患者冠脉血管狭窄程度,分析mtDNA含量与病变血管支数、Gensini评分的关系。结果 ACS组患者mtD-NA含量低于对照组(P0.001)。根据病变血管支数分组后,单支、双支、三支血管病变组mtDNA含量均低于对照组(P0.05),双支、三支血管病变组mtDNA含量均低于单支血管病变组(P0.05)。根据Gensi-ni评分结果将所有冠脉造影者分为4组,结果显示随Gensini评分的增加,mtDNA含量下降(P0.01)。将Gensini评分及mtDNA含量进行线性回归分析显示,Gensini评分与mtDNA含量呈负相关(r=-0.644,P0.001)。ROC曲线分析显示,mtDNA含量用于诊断ACS的曲线下面积为0.855(P0.001),敏感度及特异度分别为0.756和0.833。结论 MtDNA含量与ACS密切相关,且与代表冠脉狭窄程度的Gensini评分相关,mtDNA含量检测在ACS诊断中有一定的应用价值。
[Abstract]:Objective to investigate the relationship between mitochondrial DNA(mitochondrial mtDNA in peripheral blood and acute coronary syndrome (ACS). Methods 244 consecutive patients who underwent coronary angiography in the affiliated Hospital of Chengde Medical College were divided into ACS group (n = 183) and control group (n = 61). The content of mtDNA in peripheral blood of two groups was measured by real-time quantitative PCR. According to the number of diseased vessels, the patients were divided into three groups: single vessel lesion group, double vessel lesion group and three vessel lesion group. To analyze the relationship between the content of mtDNA and the number of diseased vessels and the score of Gensini. Results the content of mtD-NA in ACS group was lower than that in control group (P 0.001). According to the number of vessel branches, the mtDNA content in single vessel, double vessel and three vessel lesion group was lower than that in control group (P 0.05). The mtDNA content in double vessel disease group and three vessel lesion group was lower than that in single vessel disease group. According to the Gensi-ni score, all patients were divided into 4 groups. The results showed that the content of mtDNA decreased with the increase of Gensini score. The linear regression analysis of Gensini score and mtDNA content showed that there was a negative correlation between mtDNA content and Gensini score. The curve analysis showed that the area under the curve of mtDNA content for the diagnosis of ACS was 0.855 and P0.001, and the sensitivity and specificity were 0.756 and 0.833, respectively. Conclusion the content of MtDNA is closely related to ACS, and it is valuable to detect mtDNA content in the diagnosis of ACS, which is related to the Gensini score which represents the degree of coronary artery stenosis.
【作者单位】：承德医学院附属医院心脏内科;
【基金】：国家自然科学基金(编号:81000068) 河北省引进留学人员资助项目(编号:C2015005001)
【分类号】：R541.4

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