微泡破坏促进骨髓间充质干细胞归巢治疗犬心肌梗死

发布时间：2018-05-19 20:43

本文选题：心肌梗死 + 诊断超声介导微泡破坏　；参考：《中国老年学杂志》2017年21期

【摘要】：目的通过诊断超声介导微泡破坏联合冠状动脉内移植促进骨髓间充质干细胞(BMSCs)归巢梗死心肌,为临床治疗心肌梗死探索新的方法。方法急性心肌梗死(AMI)造模成功犬随机分为4组,每组6只,造模7 d后,分别给予磷酸盐缓冲液(PBS)、超声+微泡+PBS、BMSCs、超声+微泡+BMSCs。28 d后,评估心功能和灌注缺损区域占左心室灌注区的百分比(DA%),评估心肌梗死面积占左心室心肌横断面面积的百分比,BMSCs分化的心肌细胞数量及是否出现α-肌动蛋白。结果超声+微泡+冠状动脉移植BMSCs和单纯冠状动脉移植BMSCs均可缩小心肌梗死面积和灌注缺损面积、改善心功能和室壁运动异常指数(WMSI)、促进BMSCs在心肌内存活,但前者效果更显著;两者均可表达α-肌动蛋白,但前者α-肌动蛋白染色阳性的细胞数多于后者。结论诊断超声介导微泡破坏联合冠状动脉内移植可以促进BMSCs归巢梗死心肌,使更多的BMSCs抵达梗死区域分化为有功能的心肌细胞,从而降低心肌梗死面积并改善心脏功能。
[Abstract]:Objective to explore a new method for clinical treatment of myocardial infarction by ultrasound mediated microbubble destruction combined with intracoronary transplantation to promote homing infarct myocardium of bone marrow mesenchymal stem cells (BMSCs). Methods successful acute myocardial infarction (AMI) dogs were randomly divided into 4 groups, 6 dogs in each group. After 7 days of modeling, phosphate buffer solution PBSs, ultrasound microbubbles PBSs BMSCs and ultrasound microbubbles BMSCs.28 d were given respectively. To evaluate the cardiac function and the percentage of left ventricular perfusion area in the area of perfusion defect and to evaluate the percentage of myocardial infarction area to the cross-sectional area of left ventricle, the number of myocardial cells differentiated by BMSCs and the presence of 伪 -actin were evaluated. Results Ultrasonic microbubble coronary artery transplantation (BMSCs) and coronary artery grafts (BMSCs) could reduce myocardial infarction size and perfusion defect area, improve cardiac function and ventricular wall motion abnormality index (WMSI), and promote BMSCs survival in myocardium, but the former was more effective. Both of them expressed 伪 -actin, but the number of 伪 -actin positive cells in the former was more than that in the latter. Conclusion Ultrasound-mediated microbubble destruction combined with intracoronary transplantation can promote BMSCs homing infarct myocardium and make more BMSCs arrive in the infarcted area and differentiate into functional cardiomyocytes, thus reducing myocardial infarction size and improving cardiac function.
【作者单位】：北华大学附属医院急诊科;吉林大学第一医院放射线科;吉林大学第一医院心脏外科;
【基金】：吉林省卫生计生科研计划(20152057) 吉林市医疗卫生指导性计划项目(201737056)
【分类号】：R542.22

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