硫化氢对低氧性肺动脉高压大鼠P47phox表达的影响

发布时间：2018-05-20 16:59

本文选题：硫化氢 + 肺动脉高压　；参考：《中国老年学杂志》2017年03期

【摘要】：目的观察硫化氢H2S对低氧性肺动脉高压(HPH)大鼠P47phox表达的影响。方法 36只体重(180±20)g健康成年雄性SD大鼠,随机分为常氧组、低氧组、低氧+硫氢化钠(Na HS)组,每组12只。低氧16 d后分别检测其平均肺动脉压(m PAP)、右心室肥厚情况和肺血管形态学指标的变化。采用Western印迹检测肺动脉组织P47phox的表达水平。结果与常氧组比较,低氧组m PAP明显升高,右室/(左室+室间隔)比值显著增加(P0.01);部分肌型动脉百分比明显升高,非肌型动脉百分比明显降低(P0.05);肺动脉组织P47phox表达升高(P0.05)。与低氧组比较,低氧+Na HS组m PAP、右室/(左室+室间隔)比值均明显降低(P0.01);肺小血管中肌型动脉、部分肌型动脉百分比明显降低,非肌型动脉百分比明显升高(P0.05);肺动脉组织P47phox表达明显降低(P0.01)。结论 H2S可能通过抑制大鼠肺动脉组织P47phox表达而调节低氧性肺血管结构重建和HPH的形成。
[Abstract]:Objective to observe the effect of hydrogen sulfide (H 2S) on the expression of P47phox in hypoxic pulmonary hypertension (HPH) rats. Methods Thirty-six healthy male SD rats with body weight of 180 卤20 g were randomly divided into two groups: normoxic group (n = 12) and hypoxic sodium sulphide group (n = 12). After hypoxia for 16 days, the mean pulmonary artery pressure (MPP), right ventricular hypertrophy (RV) and pulmonary vascular morphology were measured. The expression of P47phox in pulmonary artery was detected by Western blot. Results compared with the normoxic group, m PAP was significantly increased in hypoxia group, the ratio of right ventricular septum (left ventricular septum) was increased significantly, the percentage of partial muscular artery was increased significantly, the percentage of non-muscular artery was significantly decreased, and the expression of P47phox in pulmonary artery was increased (P 0.05). Compared with the hypoxic group, the ratios of m PAPand right ventricular septum (left ventricular septum) in hypoxic Na HS group were significantly lower than those in hypoxic group, and the percentage of muscular artery and partial muscular artery in small pulmonary vessels were significantly decreased. The percentage of non-muscular arteries increased significantly, and the expression of P47phox in pulmonary artery decreased significantly (P 0.01). Conclusion H2S may regulate hypoxic pulmonary vascular remodeling and HPH formation by inhibiting the expression of P47phox in rat pulmonary artery.
【作者单位】：河西学院医学院生理学与病理生理学教研室;河西学院医学院预防医学教研室;兰州大学公共卫生学院卫生毒理研究所;
【分类号】：R36

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