细胞周期蛋白A1对SKM-1细胞增殖的影响及其在骨髓异常增生综合征中的作用

发布时间：2018-05-25 13:13

本文选题：细胞周期蛋白A + 骨髓增生异常综合征　；参考：《中国实验血液学杂志》2017年06期

【摘要】：目的:通过小干扰RNA(siRNA)下调Cyclin A1的表达,观察其对骨髓增生异常综合征(MDS)细胞系SKM-1细胞增殖的影响并探讨其作用机制。方法:采用阳离子脂质体转染方法,在SKM-1细胞中转染Cyclin A1的siRNA;转染48 h后收集细胞,应用Western blot及RT-PCR测定转染效率;应用CCK-8方法检测细胞增殖,Western blot及RT-PCR检测Cyclin A1基因沉默前后CDK2、RUNX1、SRSF2基因表达的水平。结果:转染Cyclin A1特异性siRNA 48 h后,Cyclin A1蛋白及mRNA表达水平显著下降(P0.01)。下调Cyclin A1后,SKM-1细胞的增殖受抑,CDK2、RUNX1及SRSF2基因的蛋白及mRNA表达量均下调(P0.05)。结论:Cyclin A1表达下调后,SKM-1细胞的增殖受到抑制,表明Cyclin A1可能是MDS治疗的潜在靶点之一。
[Abstract]:Aim: to investigate the effect of small interfering RNA-siRNAs (siRNAs) on the proliferation of Cyclin A1 cell line SKM-1 cells and its mechanism. Methods: the siRNAs of Cyclin A1 were transfected into SKM-1 cells by cationic liposome transfection, and the transfection efficiency was measured by Western blot and RT-PCR after 48 h transfection. CCK-8 and RT-PCR were used to detect the expression of CDK2 / RUNX1 / SRSF2 gene before and after Cyclin A1 gene silencing. Results: the expression of Cyclin A1 protein and mRNA decreased significantly 48 h after transfection of Cyclin A1 specific siRNA. After down-regulation of Cyclin A1, the proliferation of SKM-1 cells was inhibited and the protein and mRNA expression of RUNX1 and SRSF2 genes were down-regulated. Conclusion the proliferation of SKM-1 cells is inhibited after the down-regulation of the expression of Cyclin A1, which suggests that Cyclin A1 may be one of the potential targets of MDS therapy.
【作者单位】：北京大学人民医院北京大学血液病研究所;北京大学国际医院血液科;
【基金】：北京市自然科学基金(7122190) 教育部留学回国人员科研启动基金(教司留-贾晋松)
【分类号】：R551.3

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