尼可地尔抑制野百合碱诱导的肺动脉高压小鼠肺血管重构

发布时间：2018-05-28 03:25

本文选题：肺动脉高压 + ATP敏感性钾离子通道　；参考：《南京医科大学学报(自然科学版)》2017年08期

【摘要】：目的 :研究ATP敏感性钾离子通道开放剂尼可地尔对野百合碱诱导的肺动脉高压小鼠肺血管重构的影响。方法 :CD1雄性小鼠随机分为对照组、野百合碱模型组、野百合碱+尼可地尔治疗组,每组6只,后2组给予野百合碱(400 mg/kg)皮下注射,每周1次,连续8周,制备肺动脉高压模型,治疗组每日给予尼可地尔(10.5 mg/kg)灌胃。8周后通过直接经膈肌右心室穿刺法测定右心室收缩压;称重法计算右心肥厚指数;HE染色观察右室心肌细胞形态;弹力纤维染色测定肺小动脉中膜厚度;免疫组化检测肺动脉平滑肌细胞α-肌动蛋白(α-smooth muscle actin,α-SMA)表达并计算肺小动脉肌化程度。结果:尼可地尔(10.5 mg/kg)能显著降低野百合碱诱导的小鼠右心室收缩压升高(P0.01)和右心肥厚(P0.05),减轻肺小动脉中膜增厚(P0.01),抑制肺小动脉完全肌化(P0.01),减轻右心心肌细胞损伤。结论 :尼可地尔能通过抑制肺血管重构减轻野百合碱诱导的肺动脉高压,其可能是一种能有效防治肺动脉高压的药物。
[Abstract]:Objective: To study the effect of ATP sensitive potassium channel opener nicorandil on pulmonary vascular remodeling in pulmonary hypertension induced by monocrotaline. Methods: CD1 male mice were randomly divided into control group, monocrotaline model group, monocrotaline + nicorandil treatment group, 6 rats in each group, and 2 groups were given subcutaneous injection of monocrotaline (400 mg/kg), each group was given subcutaneous injection. The pulmonary hypertension model was prepared for 8 weeks on week 1, and the treatment group was given the right ventricular systolic pressure by direct phrenic right ventricle puncture after.8 weeks. The right ventricular hypertrophy index was calculated by weighing method, the right ventricular myocyte shape was observed by HE staining, and the thickness of the middle pulmonary artery in the pulmonary artery was measured by HE staining. The expression of alpha actin (alpha -smooth muscle actin, alpha -SMA) in pulmonary artery smooth muscle cells was detected by histochemistry and the degree of pulmonary arteriolating was calculated. Results: nicorandil (10.5 mg/kg) could significantly reduce the right ventricular systolic pressure (P0.01) and right heart thickness (P0.05) induced by nilrotaline, reduce the thickening of the pulmonary arterioles (P0.01) and reduce the lung small size of the pulmonary arterioles. Conclusion: Nicorandil can reduce pulmonary arterial hypertension induced by monocrotaline by inhibiting pulmonary vascular remodeling, and it may be an effective drug to prevent pulmonary hypertension.
【作者单位】：南京医科大学第一附属医院呼吸与危重症学科;
【基金】：国家自然科学基金(81273571)
【分类号】：R544.1

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