硫化氢通过抑制STAT1通路改善高甲状腺素导致的大鼠心肌纤维化

发布时间：2018-05-29 17:19

本文选题：硫化氢 + 甲状腺功能亢进性心肌病　；参考：《临床心血管病杂志》2017年08期

【摘要】：目的:观察硫化氢对高甲状腺素导致的大鼠心肌纤维化及基质金属蛋白酶14(MMP14)、MMP16和转录激活因子1(STAT1)蛋白表达的影响。方法:取SD雄性大鼠随机分为4组:对照组(C组),甲亢性心肌组(L-Thy组),硫化氢+甲亢性心肌组(H2S+L-Thy组),硫化氢干预组(H2S组)。经腹腔注射左旋甲状腺激素(Left thyroid hormone,L-Thy)建立甲状腺功能亢进大鼠模型,C组相同步骤每日给予等量0.9%氯化钠,H2S+L-Thy组和H2S组经腹腔注射硫氢化钠溶液;Masson染色观察心肌纤维化及计算心肌胶原容积分数(Collagen volume fraction,CVF);Western blotting检测MMP14、MMP16和STAT1蛋白的表达。结果:与C组相比,LThy组心肌细胞紊乱,心肌纤维沉积明显增多、CVF增高(P0.05),心肌MMP14、MMP16以及STAT1蛋白的表达均上调(P0.05);而与L-Thy组相比,H2S+L-Thy组大鼠心肌组织纤维沉积明显减少、CVF下降(P0.05),MMP14、MMP16以及STAT1的表达均有所下调(P0.05)。结论:硫化氢可能通过STAT1通路下调MMP14和MMP16表达水平,并改善高甲状腺素导致的大鼠心肌纤维化。
[Abstract]:Aim: to investigate the effects of hydrogen sulfide on myocardial fibrosis and the expression of matrix metalloproteinase-14 (MMP14) MMP16 and activator 1 (STAT1) protein in hyperthyroxine induced myocardial fibrosis in rats. Methods: Sprague-Dawley male rats were randomly divided into 4 groups: control group C, hyperthyroidism myocardium group L-Thy group, hydrogen sulfide hyperthyroidism myocardial group (H 2S L-Thy) group, hydrogen sulfide intervention group (H 2S group). The rat model of hyperthyroidism was established by intraperitoneal injection of leeft thyroid hormoney L-Thy. at the same step, the rats in group C were given the same amount of 0.9% sodium chloride H2S L-Thy and group H 2S to observe myocardial fiber by intraperitoneal injection of sodium sulphide solution. The expression of MMP14MMP16 and STAT1 protein was detected by Western blotting. Results: compared with group C, myocardial cells in LThy group were disordered. The expression of MMP14MMP16 and STAT1 protein were up-regulated in myocardium, and the expression of MMP14MMP16 and STAT1 protein were significantly decreased in H2S L-Thy group compared with that in L-Thy group, and the expression of MMP14MMP16 and STAT1 were down-regulated in H2S L-Thy group compared with that in L-Thy group, but the expression of MMP14MMP16 and MMP14MMP16 were down-regulated in H2S L-Thy group compared with that in L-Thy group, and the expression of MMP14MMP16 and MMP16 were down-regulated in H2S L-Thy group. Conclusion: hydrogen sulfide may down-regulate the expression of MMP14 and MMP16 through STAT1 pathway and improve myocardial fibrosis induced by hyperthyroxine in rats.
【作者单位】：南华大学附属第一院心血管内科;南华大学附属第二医院药剂科;
【基金】：国家自然科学基金项目(No:81270181;81202830)
【分类号】：R542.2

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