Obestatin在心肌梗死后心力衰竭大鼠循环和肾脏的表达及其生物学意义

发布时间：2018-05-31 04:53

本文选题：obestatin + 心力衰竭　；参考：《中国现代医学杂志》2017年01期

【摘要】：目的探究obestatin在心肌梗死后心力衰竭(以下简称心衰)大鼠循环和肾脏组织中的表达及其可能的生物学意义。方法以冠状动脉结扎法复制大鼠心肌梗死后心衰模型,酶联免疫吸附法检测大鼠血浆obestatin浓度,免疫组织化学法检测obestatin在肾脏组织的表达并分析累积光密度值,比较不同组的差异,Western blot检测不同组肾脏组织表达obestatin水平,并进行半定量分析。结果心衰组大鼠循环血浆内obestatin浓度较正常组及假手术组增高(P0.05),免疫组织化学法显示,obestatin在大鼠肾脏肾小管和集合管表达,心衰组大鼠肾脏组织obestatin表达较正常组和假手术组升高(P0.05)。结论 Obestatin在心力衰竭大鼠循环和肾脏表达增高,可能以内分泌及自分泌或旁分泌形式参与心衰水潴留的发生、发展。
[Abstract]:Objective to investigate the expression of obestatin in circulatory and renal tissues of rats with heart failure after myocardial infarction and its possible biological significance. Methods Rat heart failure model was established by coronary artery ligation, plasma obestatin concentration was detected by enzyme-linked immunosorbent assay (Elisa), expression of obestatin in renal tissue was detected by immunohistochemistry and cumulative optical density was analyzed. The expression of obestatin in renal tissues of different groups was detected by Western blot and semi-quantitative analysis was performed. Results the level of obestatin in circulating plasma in CHF group was higher than that in normal group and sham operation group (P 0.05). Immunohistochemical method showed that OBESTatin was expressed in renal tubules and collecting ducts of rats, and the expression of obestatin in kidney tissue of CHF group was higher than that of normal group and sham operation group (P 0.05). Conclusion the increased expression of Obestatin in circulation and kidney of rats with heart failure may be involved in the occurrence and development of water retention in heart failure in the form of endocrine autocrine or paracrine.
【作者单位】：第二军医大学长海医院心内科;
【基金】：国家自然科学基金项目(No:81470517)
【分类号】：R542.22;R541.6

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