再生障碍性贫血患者骨髓单个核细胞端粒长度及P53、P21的表达

发布时间：2018-06-01 00:37

本文选题：端粒长度 + P　；参考：《中国病理生理杂志》2017年03期

【摘要】：目的:研究再生障碍性贫血(AA)患者骨髓单个核细胞(BMMNC)的端粒长度以及P53和P21表达水平,探讨它们与AA发病的关系。方法:采用实时荧光定量聚合酶链反应(RT-qPCR)检测60例AA患者[其中非重型再障(NSAA)38例,重型再障(SAA)22例]和25例对照者BMMNC中端粒长度以及P53和P21的mRNA表达情况;采用Western blot法检测P53和P21蛋白表达情况;并进行相关性比较。骨髓活检术检测骨髓造血细胞成分分布情况;流式细胞术检测CD34~+细胞占有核细胞百分比情况。结果:AA患者端粒长度较对照组明显缩短骨髓造血细胞成分及CD34~+细胞占有核细胞百分比较对照组明显减少(P0.05);NSAA、SAA患者与对照组相比端粒长度均明显缩短,骨髓造血细胞成分及CD34~+细胞占有核细胞百分比均明显减少(P0.05);SAA与NSAA比较,端粒长度明显缩短,骨髓造血细胞成分及CD34~+细胞占有核细胞百分比明显减少(P0.05)。AA患者P53和P21的mRNA及蛋白表达水平均较对照组明显升高(P0.05);NSAA和SAA患者P53和P21的mRNA及蛋白表达水平与对照组相比均明显升高(P0.05);SAA患者P53和P21的mRNA及蛋白表达水平较NSAA明显升高(P0.05)。端粒长度与P53表达水平或P21表达水平之间没有相关性(P0.05);P53表达水平与P21表达水平之间呈正相关(P0.05)。结论:端粒长度的改变以及P53和P21可能参与了再障的发病经过,推测可能是通过抑制造血干细胞的增殖和分化,从而引发造血细胞凋亡。
[Abstract]:Aim: to study the telomere length and the expression levels of p53 and P21 in bone marrow mononuclear cells (BMMNCs) of patients with aplastic anemia (AA) and to explore their relationship with the pathogenesis of AA. Methods: Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the telomere length and the expression of p53 and P21 mRNA in BMMNC of 60 AA patients (38 cases of non-severe aplastic anemia, 22 cases of severe aplastic anemia) and 25 cases of controls. The expression of p53 and P21 protein was detected by Western blot, and the correlation was compared. Bone marrow biopsy was performed to detect the distribution of hematopoietic components, and flow cytometry was used to detect the percentage of CD34 ~ cells in nuclear cells. Results the length of telomere in the patients with 10% AA was significantly shorter than that in the control group. The percentage of hematopoietic cells and the percentage of nuclear cells occupied by CD34 ~ cells were significantly reduced in the patients with small bone marrow hematopoietic cells than in the control group. The telomere length of the patients with NSAA SAA was significantly shorter than that of the control group. Bone marrow hematopoietic cell components and percentage of CD34 ~ cells occupied nuclear cells were significantly decreased compared with NSAA, telomere length was significantly shortened. Bone marrow hematopoietic components and percentage of CD34 ~ cells in nuclear cells decreased significantly the expression levels of mRNA and protein of p53 and P21 in patients with P0.05A and AA were significantly higher than those in controls. The mRNA and protein expression levels of p53 and P21 in patients with P0.05A and SAA were significantly higher than those in patients with P0.05A and SAA. The mRNA and protein expression levels of p53 and P21 in patients with P0.05A were significantly higher than those in NSAA patients. There was no correlation between telomere length and p53 expression level or P21 expression level. Conclusion: the changes of telomere length and p53 and P21 may be involved in the pathogenesis of aplastic anemia, which may be induced by inhibiting the proliferation and differentiation of hematopoietic stem cells and inducing hematopoietic cell apoptosis.
【作者单位】：山东中医药大学附属医院血液科;
【基金】：国家自然科学基金青年基金资助项目(No.81303080) 山东省中医药科技发展计划资助项目(No.2013ZDZK-037) 山东省科技发展计划资助项目(No.2014GSF119002)
【分类号】：R556.5

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