稳定性心绞痛患者SIRT1表达与氧化应激的相关性研究

发布时间：2018-06-01 04:52

本文选题：稳定性心绞痛 + SIRT　；参考：《南京医科大学学报(自然科学版)》2017年05期

【摘要】：目的:检测冠心病稳定性心绞痛患者外周血单个核细胞(PBMC)中沉默信息调节因子2相关酶1(SIRT1)蛋白表达和血清丙二醛(MDA)、超氧化物歧化酶(SOD)水平,探讨SIRT-1和氧化应激的相关性。方法:选择稳定性心绞痛患者25例,并与20名健康者(对照组)做比较。采用Western blot法检测PBMC中SIRT1蛋白表达和血清MDA、SOD水平,分析SIRT1蛋白表达和氧化应激相关性。结果:与对照组相比,稳定性心绞痛患者PBMC中SIRT1蛋白表达显著降低(P0.05);血清MDA升高,SOD降低(P均0.05);Pearson直线相关法分析稳定性心绞痛患者的SIRT1蛋白表达水平与血清MDA呈显著负相关(r=-0.539,P0.05),与SOD呈正相关(r=0.630,P0.05)。结论:冠心病稳定性心绞痛患者SIRT1蛋白表达水平降低,与血清MDA呈负相关,与SOD呈正相关,提示SIRT1与稳定性心绞痛患者机体内氧化应激关系密切。
[Abstract]:Aim: to detect the expression of silencing signal regulatory factor-2 related enzyme 1 sirt1 (sir1) and the level of serum malondialdehyde (MDA) MDAD (superoxide dismutase) in peripheral blood mononuclear cells (PBMC) of patients with stable angina pectoris (CHD), and to explore the correlation between SIRT-1 and oxidative stress. Methods: 25 patients with stable angina pectoris were selected and compared with 20 healthy people (control group). The expression of SIRT1 protein in PBMC and the level of serum MDA-SOD were detected by Western blot method. The correlation between the expression of SIRT1 protein and oxidative stress was analyzed. Results: compared with the control group, The expression of SIRT1 protein in PBMC of patients with stable angina pectoris decreased significantly (P 0.05), and the expression of SIRT1 protein in patients with stable angina pectoris was negatively correlated with serum MDA by Pearson linear correlation method, and was positively correlated with SOD in patients with stable angina pectoris. Conclusion: the expression of SIRT1 protein in patients with stable angina pectoris of coronary heart disease is lower, negatively correlated with serum MDA, and positively correlated with SOD, suggesting that SIRT1 is closely related to oxidative stress in patients with stable angina pectoris.
【作者单位】：南京医科大学第二附属医院心内科;
【分类号】：R541.4

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