KIF4A对巨噬细胞血管生成相关因子表达的调节作用

发布时间：2018-06-05 17:48

本文选题：巨噬细胞 + 血管生成相关因子　；参考：《山东大学学报(医学版)》2017年01期

【摘要】：目的探讨驱动蛋白KIF4A对巨噬细胞不同亚群中血管生成相关因子表达的影响。方法 THP-1细胞在PM A及不同人重组细胞因子的外界刺激下分别分化为M0、M1、M2细胞,采用ELISA技术检测此3种巨噬细胞亚群中血管生成相关因子的表达水平。利用siRNA转染敲除M0、M1、M2细胞中KIF4A的表达,采用实时定量PCR检测敲除后血管生成相关因子的表达水平。结果巨噬细胞M0上清中可检测到LIF、VEGF、s VEGFR1及TIM P1,而s VEGFR2、Flt-3、Leptin、LeptinR、CD40L均不表达。其中LIF、VEGF、TIM P1在M0向M1、M2分化过程中,表达差异均无统计学意义(P0.05);s VEGFR1在M0向M1分化过程中表达差异无统计学意义(P0.05),向M2分化过程中表达显著上调(P0.01)。转染KIF4A siRNA后,M0与M1细胞中LIF、s VEGFR1、VEGF、TIM P1的表达差异均无统计学意义(P0.05);M2细胞中LIF、VEGF、TIM P1表达差异均无统计学意义(P0.05),但s VEGFR1表达水平明显降低(P0.05)。结论 M2细胞s VEGFR1表达水平显著上调;KIF4A参与M2细胞中s VEGFR1的表达。
[Abstract]:Objective to investigate the effect of KIF4A on the expression of angiogenic factors in different subsets of macrophages. Methods THP-1 cells were stimulated by PM A and different human recombinant cytokines to differentiate into M0 / M1M 2 cells respectively. The expression of angiogenesis related factors in these three macrophages subsets was detected by Elisa. The expression of KIF4A was detected by real-time quantitative PCR. The expression level of angiogenesis related factors after knockout was detected by using siRNA transfection. Results LIF-VEGFNs VEGFR1 and Tim P1 could be detected in the supernatant of macrophage M0, but no expression of LeptinRt2 or LeptinRt4 CD40L was detected in macrophage M0 supernatant. There was no significant difference in the expression of Lifen VEGFR1 during the differentiation from M0 to M1M2.There was no significant difference in the expression of VEGFR1 between M0 and M1, but the expression of P0.01 was up-regulated during the differentiation of M0 to M1M1.The expression of VEGFR1 was significantly up-regulated during the differentiation of M0 to M1, and the expression of VEGFR1 was significantly up-regulated during the differentiation of M0 to M1. After transfection of KIF4A siRNA, there was no significant difference in the expression of LIF-VEGFR1VEGFMP1 between M0 and M1 cells. There was no significant difference in the expression of LIF-VEGFT-TIMP1 in P0.05M-2 cells, but the expression level of sVEGFR1 decreased significantly. Conclusion the expression level of sVEGFR1 in M2 cells was significantly up-regulated, and KIF4A was involved in the expression of sVEGFR1 in M2 cells.
【作者单位】：山东大学齐鲁医院烧伤整形科;山东大学齐鲁医院口腔及颌面外科;山东大学齐鲁医院临床基础研究所;
【基金】：国家自然科学基金(81271105,31470885,31270971)
【分类号】：R54

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