DNMT3A与CTGF在AAC致大鼠心肌纤维化中的表达及相关性研究

发布时间：2018-06-11 21:35

本文选题：心肌纤维化 + 腹主动脉缩窄术　；参考：《安徽医科大学学报》2017年09期

【摘要】：目的探讨经腹主动脉缩窄(AAC)导致大鼠心肌纤维化中DNA甲基转移酶3A(DNMT3A)与结缔组织生长因子(CTGF)的表达及相关性。方法将60只8周龄雄性SD大鼠随机分为模型组及对照组。模型组行AAC术,对照组行假手术,术后2周处死大鼠获取血液标本,并取心脏。同时测定心重指数,采用ELISA法测量Ⅰ型胶原和CTGF在心肌组织匀浆中的含量,HE和Masson染色法观察心肌组织病理学改变;Western blot法测定DNMT3A和CTGF的表达。结果与对照组比较,模型组大鼠的心重指数显著上升,且心肌组织中Ⅰ型胶原和CTGF的含量明显增加;HE和Masson染色显示,模型组心肌组织呈现明显的胶原沉积;Western blot检测表明模型组DNMT3A、CTGF的含量水平明显高于对照组;DNMT3A和CTGF在大鼠心肌纤维化组织中的表达呈正相关性(r=0.64,P0.01)。结论 DNMT3A参与心肌纤维化的形成过程,并在其中起到一定的促进作用,可能与CTGF的升高有一定的相关性。
[Abstract]:Objective to investigate the expression of DNMT3A and CTGF in rat myocardial fibrosis induced by transabdominal aortic coarctation. Methods 60 8 week old male SD rats were randomly divided into model group and control group. AAC was performed in the model group and sham operation was performed in the control group. At the same time, the cardiac weight index was measured. The contents of type I collagen and CTGF in myocardial homogenate were measured by Elisa. The expression of DNMT3A and CTGF was detected by Western blot method and the pathological changes of myocardium were observed by Masson staining. Results compared with the control group, the cardiac weight index of the model group was significantly increased, and the contents of type I collagen and CTGF in myocardial tissue were significantly increased by HE and Masson staining. The expression of DNMT3A and CTGF in model group was significantly higher than that in control group. There was a positive correlation between the expression of DNMT3A and CTGF in rat myocardial fibrosis tissue. Conclusion DNMT3A is involved in the formation of myocardial fibrosis and plays a role in promoting it, which may be related to the increase of CTGF.
【作者单位】：安徽医科大学第二附属医院心胸外科;安徽医科大学心血管病研究中心;
【基金】：国家自然科学基金(编号:81570295) 安徽省科技攻关计划项目(编号:1501041148)
【分类号】：R54

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