高血压患者亚甲基四氢叶酸还原酶基因C677T多态性与血脂异常的相关性

发布时间：2018-06-20 15:31

本文选题：亚甲基四氢叶酸还原酶 + 基因多态性　；参考：《中国动脉硬化杂志》2017年02期

【摘要】：目的探讨血浆同型半胱氨酸(Hcy)及其代谢关键酶亚甲基四氢叶酸还原酶(MTHFR)C677T基因多态性与中国汉族人群原发性高血压患者血脂异常的关系。方法选取原发性高血压患者312例,女性134例,男性178例,平均年龄58.25岁,收集患者基本资料,检测血浆Hcy、血脂、空腹血糖(FBG)、尿酸(UA)等临床生物化学指标。应用Taqman探针技术检测患者MTHFR C677T基因分型。根据2007年中国成人血脂异常防治指南,将入选的312例原发性高血压患者分为血脂异常组194例和血脂正常组118例,同时将血脂异常组分为4个亚组:高胆固醇血症组54例,高甘油三酯血症组53例,混合型高脂血症组59例,低高密度脂蛋白血症组22例,并进行亚组分析。结果血脂异常组体质指数(BMI)、FBG、UA高于血脂正常组(P0.01)。血脂异常组MTHFR C677T的CC、CT、TT三种基因型频率和C、T两种等位基因频率与血脂正常组比较差异无统计学意义(P0.05),血脂异常各亚组之间的基因型频率、等位基因频率差异无统计学意义,与血脂正常组之间差异亦无统计学意义。MTHFR C677T不同基因型间四项血脂水平均无统计学差异,但TT基因型者Hcy水平高于CT、CC基因型者(P0.05),而CT基因型者Hcy水平与CC基因型者比较差异无统计学意义(P0.05)。高密度脂蛋白胆固醇(HDLC)水平与Hcy水平呈负相关(r=-0.116,P0.05),但是在校正了年龄、BMI、UA、FBG后此相关性消失;总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDLC)水平与Hcy水平无相关性(P0.05)。Logistic回归分析显示,在校正了BMI、FBG、UA后基因型与血脂异常无相关性(P0.05)。结论在原发性高血压患者中MTHFR C677T基因多态性与血脂异常无关联,而与Hcy呈显著相关。血脂异常与FBG、UA、BMI有关,而与Hcy无关,但HDLC与血浆Hcy水平呈负相关。
[Abstract]:Objective to investigate the relationship between plasma homocysteine (Hcyn) and methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism and dyslipidemia in essential hypertension patients of Han nationality in China. Methods 312 patients with essential hypertension, 134 women and 178 men, with an average age of 58.25 years, were selected. The basic data of the patients were collected and the clinical biochemical indexes such as plasma Hcycy, blood lipid, fasting blood glucose, FBGG and UAA were measured. MTHFR C677T genotyping was detected by Taqman probe technique. According to the guidelines for the prevention and treatment of adult dyslipidemia in China in 2007, 312 patients with essential hypertension were divided into two groups: 194 patients with dyslipidemia and 118 patients with normal blood lipids. At the same time, the dyslipidemia group was divided into four subgroups: 54 patients with hypercholesterolemia. There were 53 cases of hypertriglyceridemia group, 59 cases of mixed hyperlipidemia group and 22 cases of low density lipoproteinemia group. Results the body mass index (BMI) of the dyslipidemia group was higher than that of the normal blood lipid group (P 0.01). There was no significant difference in three genotypes and two alleles between MTHFR C677T and normal blood lipid group (P 0.05), but there was no significant difference in genotype frequency and allele frequency between subgroups with dyslipidemia. There was no significant difference between MTHFR C677T and normal blood lipids. There was no significant difference among the four genotypes of MTHFR C677T. However, the level of Hcy in TT genotype was higher than that in CTN CC genotype, but there was no significant difference in Hcy level between CT genotype and CC genotype. High density lipoprotein cholesterol (HDLC) level and Hcy level were negatively correlated with Hcy, but the correlation disappeared after adjusting for age BMIU UABG, but there was no correlation between total cholesterol TCU, triglyceride TGG, low density lipoprotein cholesterol (LDLCc) level and Hcy level (P0.05n.Logistic regression analysis showed that there was no correlation between HDLC level and Hcy level. There was no correlation between genotype and dyslipidemia after correcting BMIA FBGG UA (P 0.05). Conclusion MTHFR C677T gene polymorphism is not associated with dyslipidemia in patients with essential hypertension, but is significantly correlated with Hcy. Dyslipidemia was associated with BMI, but not with Hcy, but HDLC was negatively correlated with plasma Hcy level.
【作者单位】：山东中医药大学;山东大学附属千佛山医院心内二科;
【基金】：山东省科技发展计划项目(2014GSF118055) 山东省医药卫生科技发展计划项目(2013WS0130)
【分类号】：R544.1

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