基质金属蛋白酶9及其组织型抑制因子1在高血压大鼠血液及组织中的变化

发布时间：2018-06-24 15:37

  本文选题：肾血管性高血压大鼠 + 基质金属蛋白酶　； 参考：《中国实验动物学报》2017年02期

【摘要】：目的探讨肾血管性高血压大鼠(RHR)血清、脑及血管组织中基质金属蛋白酶9(MMP-9)和组织型基质金属蛋白酶抑制因子1(TIMP-1)的表达以及血压与两者的关系。方法雄性SD大鼠80只随机分成RHR组(40只)和假手术组(40只)。RHR组采用双肾-双夹法制作高血压大鼠模型,鼠尾测压仪测定血压。根据Longa5评分法和病理学结果确定脑卒中;Western blot和免疫组化测定脑组织和血管中MMP-9和TIMP-1的表达,双抗体夹心ELISA检测血清MMP-9和TIMP-1水平。结果与假手术组比较,RHR组大鼠术后2、4、6、8、10、12周血压均明显升高[(157±9.0)比(128±7.0),(176±10.0)比(122±6.0),(194±8.0)比(117±6.5),(202±12.0)比(124±8.0),(218±15.0)比(126.±8.5),(224±20.0)比(129.±9.0)mm Hg,均P0.05];术后12周,RHR大鼠血清MMP-9高于假手术组[(783.4±109.79)比(573.4±109.59)ng/m L,P0.05];而RHR大鼠血清TIMP-1低于假手术组[(313.02±83.9)比(976.19±191.1)pg/m L,P0.05]。同时,RHR大鼠脑组织和血管MMP-9的表达均明显高于假手术组(均P0.05),而TIMP-1的表达则均明显低于假手术组(均P0.05)。Pearson相关分析显示收缩压与血清、脑及血管组织中的MMP-9水平均呈正相关(r=0.557,r=0.774和r=0.661,均P0.05),而与TIMP-1水平均呈负相关(r=-0.481,r=-0.535和r=-0.685,均P0.01)。结论肾血管性高血压大鼠血清、脑和血管组织中MMP-9表达升高,而TIMP-1表达降低。两者在血液和组织中的变化趋势保持一致,血压的升高与血液和组织中MMP-9的升高和TIMP-1的降低相关。
[Abstract]:Objective to investigate the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in serum, brain and vascular tissues of renovascular hypertensive rats (RHR) and the relationship between blood pressure and the expression of MMP-9 and TIMP-1. Methods 80 male Sprague-Dawley rats were randomly divided into two groups: RHR group (n = 40) and sham operation group (n = 40). The expressions of MMP-9 and TIMP-1 in brain tissue and blood vessel were determined by immunohistochemistry and Western blot according to the results of Longa5 score and pathology. The levels of MMP-9 and TIMP-1 in serum were detected by double antibody sandwich Elisa. 缁撴灉涓庡亣鎵嬫湳缁勬瘮杈，

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