miR-206对apoE-KO动脉粥样硬化小鼠肝脏X受体α表达的影响

发布时间：2018-07-04 14:49

  本文选题：miR-206 + 肝脏X受体α　； 参考：《山西医科大学》2015年硕士论文

【摘要】：目的:本实验通过构建apo E-KO小鼠动脉粥样硬化模型,探讨mi R-206对apo E-KO动脉粥样硬化小鼠肝脏胆固醇逆转运相关蛋白肝脏X受体α表达和胆固醇流出率的影响,进一步研究mi R-206参与胆固醇逆转运的机制。方法:高脂饮食喂养apo E-KO小鼠30只,建立动脉粥样硬化模型。将小鼠随机平均分为3组,A组:PBS对照组;B组:mi R-206 mimic组;C组:mi R-206 inhibitor组。分别于腹腔皮下注射PBS溶液、mi R-206 mimic溶液、mi R-206 inhibitor溶液。4周后,眶静脉取血检测血脂水平,取主动脉根部HE染色及油红O染色观察动脉粥样硬化程度,荧光定量RT-PCR和Western-blot法检测各组小鼠肝脏m RNA相对表达量和蛋白含量,液态闪烁计数法检测各组小鼠腹腔巨噬细胞胆固醇的流出率。结果:mi R-206 mimic组较对照组HDL-C水平升高;与对照组、mi R-206 mimic组相比,mi R-206 inhibitor组TC、LDL均显著升高,HDL明显下降。各组TG无明显不同。主动脉根部行HE染色、油红O染色可见对照组粥样硬化程度较轻,mi R-206inhibitor组动脉粥样硬化程度最为严重,mi R-206 mimic组动脉粥样硬化病变极其微小。与对照组相比,mi R-206 mimic组肝LXRαm RNA和蛋白表达增多,mi R-206inhibitor组表达减少。胆固醇流出率的结果显示,mi R-206 mimic组百分率的较对照组的明显升高,mi R-206 inhibitor组较对照组明显下降。结论:mi R-206与LXRα之间可能有中间物质的参与,构成了反馈环,作为LXR活性自身调控的一部分,共同调节胆固醇逆转运的过程。
[Abstract]:Aim: to investigate the effects of miR-206 on the expression of cholesterol reverse transporter associated protein X receptor 伪 and cholesterol efflux rate in the liver of apo E-KO mice by establishing an atherosclerosis model of apo E-KO mice. To further study the mechanism of mi R-206 involved in cholesterol reverse transport. Methods: 30 apo E-KO mice were fed with high fat diet to establish atherosclerosis model. The mice were randomly divided into three groups: group A, control group, group B, group C, group 鈪，

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