致动脉粥样硬化过程中不同表型的巨噬细胞对泡沫细胞形成的影响及机制

发布时间：2018-07-04 17:34

本文选题：动脉粥样硬化 + 巨噬细胞　；参考：《中西医结合心脑血管病杂志》2015年11期

【摘要】：目的探讨致动脉粥样硬化过程中不同表型的巨噬细胞对泡沫细胞形成的影响及机制。方法提取C57BL/6小鼠骨髓单核细胞诱导分化为巨噬细胞,分别给予干扰素γ(IFN-γ)、内毒素(LPS)、白细胞介素4(IL-4)刺激分化为M1和M2型巨噬细胞,加入氧化型低密度脂蛋白(ox-LDL)孵育48 h。高效液相色谱法(HPLC)检测细胞内的总胆固醇(TC)、游离胆固醇(FC)及胆固醇酯(CE)的含量。行RT-PCR及Westernblot检测巨噬细胞清道夫受体CD36及SR-A1在mRNA和蛋白水平的表达;行Westernblot检测内质网应激(ERS)的标志性分子糖调节蛋白78(GRP78)和GRP94的表达。结果 M1型巨噬细胞组油红O染色阳性细胞较M2型巨噬细胞组少,M1型巨噬细胞内TC、FC及CE的含量较M2型巨噬细胞低(P0.05)。清道夫受体CD36及SR-A1在M2型巨噬细胞的表达高于M1型巨噬细胞,内质网应激的标志性分子糖调节蛋白78和糖调节蛋白94的表达在M2型巨噬细胞高于M1型巨噬细胞。结论在ox-LDL刺激下M2型巨噬细胞较M1型巨噬细胞更易形成泡沫细胞,其机制可能是由于内质网应激增加了清道夫受体CD36及SR-A1在M2型巨噬细胞的表达,促进了M2型巨噬细胞对ox-LDL的吞噬。
[Abstract]:Objective to investigate the effect and mechanism of macrophages with different phenotypes on foam cell formation in atherogenic process. Methods the bone marrow monocytes of C57BL / 6 mice were induced to differentiate into macrophages. Interferon 纬 (IFN- 纬), endotoxin (LPS) and interleukin-4 (IL-4) were used to induce macrophage differentiation into M1 and M2 macrophages. The macrophages were incubated with oxidized low density lipoprotein (ox-LDL) for 48 h. The contents of total cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE) were determined by high performance liquid chromatography (HPLC). The expressions of CD36 and SR-A1 mRNA and protein in macrophages were detected by RT-PCR and Western blot, and the expression of glycomodulin 78 (GRP78) and GRP94, the iconic molecules of endoplasmic reticulum stress (ERS), were detected by Western blot. Results the oil red O staining positive cells in M1 type macrophage group were lower than those in M2 type macrophage group (P0.05), and the contents of TCU FC and CE in M1 type macrophage were lower than those in M2 type macrophage group (P0.05). The expression of scavenger receptor CD36 and SR-A1 in M2 macrophages was higher than that in M1 macrophages, and the expression of glycomodulin 78 and glucose regulated protein 94 in M2 macrophages was higher than that in M1 macrophages. Conclusion macrophages of M2 type are more likely to form foam cells than M1 type macrophages stimulated by ox-LDL. The mechanism may be that endoplasmic reticulum stress increases the expression of scavenger receptor CD36 and SR-A1 in M2 macrophages. The phagocytosis of ox-LDL by M 2 macrophages was promoted.
【作者单位】：山西医科大学第一临床医院;山西省人民医院;山西医科大学;
【基金】：山西省科学技术厅项目(No.2014011043-5)
【分类号】：R543.5

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