米力农对LPS诱导下人冠状动脉内皮细胞炎症反应的影响

发布时间：2018-07-10 05:54

本文选题：米力农 + HCAEC　；参考：《中国免疫学杂志》2017年11期

【摘要】：目的:观察米力农对LPS诱导下人冠状动脉内皮细胞(HCAEC)炎症反应及NF-κB的影响。方法:体外培养HCAEC,分为正常组、LPS组(1μg/ml)、米力农50μmol/L组和米力农200μmol/L组,后两组均与LPS共同孵育24 h。分别采用qRT-PCR和ELISA检测IL-6、IL-8、TNF-α的mRNA和蛋白水平,分别采用Western blot和免疫荧光法检测NF-κB活化和核移位。结果:与LPS组相比,米力农显著降低HCAEC的IL-6、IL-8、TNF-α的mRNA和蛋白表达;抑制NF-κB活化和核移位(P0.05),且呈一定量效关系。结论:米力农可通过抑制NF-κB活化而降低LPS诱导的HCAEC释放炎症因子。
[Abstract]:Aim: to observe the effects of milrinone on the inflammatory response and NF- 魏 B of human coronary artery endothelial cells (HCAEC) induced by LPS. Methods: HCAECs were divided into normal group (1 渭 g/ml), milrinone 50 渭 mol / L group and milrinone 200 渭 mol / L group in vitro. The latter two groups were co-incubated with LPS for 24 h. The mRNA and protein levels of IL-6 and IL-8 TNF- 伪 were detected by qRT-PCR and Elisa respectively. The activation and nuclear translocation of NF- 魏 B were detected by Western blot and immunofluorescence. Results: compared with LPS group, milrinone significantly decreased the mRNA and protein expression of IL-6 and IL-8 TNF- 伪 in HCAEC, inhibited the activation and nuclear translocation of NF- 魏 B in a dose-dependent manner (P0.05). Conclusion: milrinone can inhibit the activation of NF- 魏 B and reduce the release of inflammatory factors by LPS-induced HCAEC.
【作者单位】：山西医科大学汾阳学院临床医学系;山西医科大学;
【基金】：山西省高等学校科技项目(No.21021005)
【分类号】：R541.6

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