阿托伐他汀对慢性兔房颤模型心房电重构的影响
发布时间：2018-07-16 16:00
【摘要】：目的:通过快速起搏心房3周建立慢性兔房颤模型,探讨阿托伐他汀(ATO)对该模型心房电重构的影响及其可能机制。方法:将24只新西兰大白兔开胸,于左心房植入起搏和测试电极,随机分为3组:模型(model)组和ATO组持续心房起搏3周,分别给予安慰剂和阿托伐他汀2.5 mg·kg~(-1)·d~(-1)灌胃;假手术(sham)组不起搏,不给药。起搏前后用电生理刺激仪检测心率、P波宽度、心房有效不应期(AERP)和房颤诱发率的变化;起搏后采用Western blot检测心房Cav1.2、Kv4.3和髓过氧化物酶(MPO)的蛋白表达水平。结果:Sham组、model组和ATO组分别有0、5和4只兔诱发持续性房颤。起搏3周后,与sham组相比,model组和ATO组兔心率和P波宽度均增加,AERP缩短(P0.05);ATO组与model组相比,AERP增加(P0.05),心率和P波宽度无明显变化。与sham组相比,model组和ATO组兔心房Cav1.2和Kv4.3的蛋白表达水平下降,MPO的蛋白表达水平升高(P0.05);ATO组与model组相比,Cav1.2的表达增加,MPO的表达下降(P0.05),Kv4.3无明显变化。结论:阿托伐他汀能够通过抑制慢性兔房颤模型心房Cav1.2蛋白表达下降和AERP的缩短,抑制心房电重构,其潜在机制可能是阿托伐他汀抑制了心房MPO蛋白的高表达。
[Abstract]:Aim: to investigate the effect of Atto vastatin (ATO) on atrial electrical remodeling in rabbit atrial fibrillation model with rapid atrial pacing for 3 Zhou Jianli. Methods: Twenty-four New Zealand white rabbits were divided into three groups randomly: the model group and the ATO group were given placebo and Atto vastatin 2.5 mg kg ~ (-1) d ~ (-1) for 3 weeks of continuous atrial pacing. No pacing or medication was given in (sham) group. The changes of heart rate P wave width, atrial effective refractory period (AERP) and atrial fibrillation evoked rate were detected by electrophysiological stimulator before and after pacing, and the protein expression of Cav1.2 Kv4.3 and myeloperoxidase (MPO) were detected by Western blot after pacing. Results 5 rabbits and 4 rabbits in the control group and ATO group induced persistent atrial fibrillation, respectively. After 3 weeks of pacing, compared with sham group, the heart rate and P-wave width of model group and ATO group increased significantly (P0.05), while the heart rate and P-wave width of ATO group increased compared with model group (P0.05), but the heart rate and P-wave width did not change significantly. Compared with sham group and ATO group, the expression level of Cav1.2 and Kv4.3 in rabbit atrium were decreased (P0.05). Compared with model group, the expression of Cav1.2 increased in ATO group (P0.05). Conclusion: Atto can inhibit atrial electrical remodeling by inhibiting the decrease of atrial Cav1.2 protein expression and the shortening of atrial electrical remodeling in chronic rabbit atrial fibrillation model. The underlying mechanism may be that Atto vastatin inhibits the high expression of atrial Cav1.2 protein.
【作者单位】： 河北省人民医院心内科;河北省人民医院肾内科;
【分类号】：R-332;R541.75
本文编号：2126894
[Abstract]:Aim: to investigate the effect of Atto vastatin (ATO) on atrial electrical remodeling in rabbit atrial fibrillation model with rapid atrial pacing for 3 Zhou Jianli. Methods: Twenty-four New Zealand white rabbits were divided into three groups randomly: the model group and the ATO group were given placebo and Atto vastatin 2.5 mg kg ~ (-1) d ~ (-1) for 3 weeks of continuous atrial pacing. No pacing or medication was given in (sham) group. The changes of heart rate P wave width, atrial effective refractory period (AERP) and atrial fibrillation evoked rate were detected by electrophysiological stimulator before and after pacing, and the protein expression of Cav1.2 Kv4.3 and myeloperoxidase (MPO) were detected by Western blot after pacing. Results 5 rabbits and 4 rabbits in the control group and ATO group induced persistent atrial fibrillation, respectively. After 3 weeks of pacing, compared with sham group, the heart rate and P-wave width of model group and ATO group increased significantly (P0.05), while the heart rate and P-wave width of ATO group increased compared with model group (P0.05), but the heart rate and P-wave width did not change significantly. Compared with sham group and ATO group, the expression level of Cav1.2 and Kv4.3 in rabbit atrium were decreased (P0.05). Compared with model group, the expression of Cav1.2 increased in ATO group (P0.05). Conclusion: Atto can inhibit atrial electrical remodeling by inhibiting the decrease of atrial Cav1.2 protein expression and the shortening of atrial electrical remodeling in chronic rabbit atrial fibrillation model. The underlying mechanism may be that Atto vastatin inhibits the high expression of atrial Cav1.2 protein.
【作者单位】： 河北省人民医院心内科;河北省人民医院肾内科;
【分类号】：R-332;R541.75
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