G-CSF对豚鼠单个缺血心房肌细胞钙、钠、钾电流的影响

发布时间：2018-07-20 15:43

【摘要】：目的:采用膜片钳全细胞记录方法,观察粒细胞集落刺激因子(G-CSF)急性干预对分离的豚鼠单个缺血心房肌细胞钙、钠、钾电流的影响。方法:使用酶解方法获得豚鼠单个心房肌细胞,在缺血缺氧条件下,采用全细胞膜片钳技术观察记录不同浓度G-CSF急性干预对钙通道电流-电压曲线、激活曲线、失活曲线,钠通道电流-电压曲线、激活曲线、失活曲线、静态失活曲线,延迟整流钾通道及其快成分、慢成分电流-电压曲线的影响。结果:随G-CSF浓度的增加,缺血心房肌细胞膜L型钙通道电流较缺血对照组有明显增大。当G-CSF浓度超过100μg/kg后,L型钙通道电流的增强维持于同一水平。当给予G-CSF浓度为300μg/kg时最大激活电压发生改变,较前有所增加。激活曲线与失活曲线未见明显改变。不同浓度G-CSF对缺血心房肌细胞膜钠离子通道电流无明显影响。G-CSF干预缺血心房肌细胞膜延迟整流钾电流未见明显改变,但延迟整流钾电流快成分在G-CSF 100μg/kg干预后明显增加,而延迟整流钾电流慢成分未见明显改变。结论:G-CSF对于缺血心房肌细胞部分通道的作用影响基本为非电压依赖性,但具有浓度依赖性,可能减少缺血心房心律失常的发生率。
[Abstract]:Aim: to investigate the effects of acute intervention of granulocyte colony stimulating factor (G-CSF) on calcium, sodium and potassium currents in isolated guinea pig atrial myocytes. Methods: isolated guinea pig atrial myocytes were obtained by enzymatic hydrolysis. Under the condition of ischemia and hypoxia, whole-cell patch clamp technique was used to observe and record the calcium channel current-voltage curve, activation curve and inactivation curve after acute intervention of different concentrations of G-CSF. The influence of sodium channel current-voltage curve, activation curve, inactivation curve, static inactivation curve, delayed rectifier potassium channel and its fast component, slow component current-voltage curve. Results: with the increase of G-CSF concentration, the L-type calcium channel current in ischemic atrial myocytes was significantly higher than that in ischemic control group. When the concentration of G-CSF exceeded 100 渭 g/kg, the enhancement of L-type calcium channel current was maintained at the same level. When the concentration of G-CSF was 300 渭 g/kg, the maximum activation voltage changed and increased. The activation curve and inactivation curve did not change obviously. Different concentrations of G-CSF had no significant effect on the sodium channel currents of ischemic atrial myocytes. The delayed rectifier potassium currents did not change significantly after G-CSF intervention, but the fast components of delayed rectifier potassium currents increased significantly after G-CSF 100 渭 g/kg intervention. But the slow composition of delayed rectifier potassium current did not change obviously. Conclusion the effect of G CSF on partial channels of ischemic atrial myocytes is voltage independent, but in a concentration dependent manner, which may reduce the incidence of ischemic atrial arrhythmias.
【作者单位】：中国人民解放军第254医院心血管内科;中国人民解放军第254医院检验科;
【分类号】：R541.7;;R329.2+4

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