非对称性二甲基精氨酸对豚鼠左心室流出道自律细胞电活动的影响
发布时间：2018-07-21 13:55
【摘要】：目的:研究内源性一氧化氮合酶(NOS)抑制物非对称性二甲基精氨酸(ADMA)对豚鼠左心室流出道自律细胞电活动的影响,探讨ADMA在源于心室流出道心律失常发生发展中的作用。方法:制备豚鼠离体左心室流出道标本,用氧饱和的改良Locke液进行恒温、恒速灌流,采用标准玻璃微电极细胞内电位记录技术记录豚鼠离体左心室流出道部位的自发慢反应电位(sAP),观测该电位对ADMA的浓度依赖性效应,ADMA分为3个浓度组(1、10和100μmol·L~(-1));用NO供体硝普钠(SNP)灌流预处理标本,分为对照组、SNP(10μmol·L~(-1))组和SNP+ADMA组,记录并分析SNP预处理对ADMA所致左心室流出道电生理效应的影响。结果:与对照组比较,1μmol·L~(-1) ADMA组sAP各项指标均无明显变化(P0.05),10和100μmol·L~(-1) ADMA组4相自动去极速度(VDD)、自发放电频率(RPF)和0相最大除极速度(Vmax)明显减慢(P0.05),复极50%和90%时间(APD50和APD90)明显延长(P0.05),100μmol·L~(-1) ADMA组动作电位幅度(APA)明显减小(P0.05);与1μmol·L~(-1) ADMA组比较,10μmol·L~(-1) ADMA组RPF、APD90及100μmol·L~(-1) ADMA组RPF、Vmax和APD50差异有统计学意义(P0.05);与10μmol·L~(-1) ADMA组比较,100μmol·L~(-1) ADMA组APA明显减小(P0.05)。10μmol·L~(-1) SNP预处理标本10 min可逆转ADMA降低左心室流出道自发放电活动的效应,与对照组比较,VDD和RPF仍明显加快(P0.05),Vmax和APA有恢复至对照组水平的趋势(P0.05),APD90明显缩短(P0.05)。结论:内源性NOS抑制物ADMA浓度依赖性降低左心室流出道自律细胞的自发放电活动,NO供体SNP预处理可逆转上述效应。
[Abstract]:Aim: to investigate the effect of asymmetric dimethyl arginine (ADMA), an endogenous nitric oxide synthase (NOS) inhibitor, on electrical activity in guinea pig left ventricular outflow tract (LVOT) autonomous cells, and to explore the role of ADMA in the development of ventricular outflow tract arrhythmias. Methods: the left ventricular outflow tract specimens of guinea pigs were prepared and perfused at constant temperature and at constant speed with oxygen-saturated modified Locke solution. The spontaneous slow response potential (SAP) of isolated left ventricular outflow tract of guinea pig was recorded by using standard glass microelectrode intracellular potential recording technique. The concentration-dependent effect of this potential on ADMA was observed. ADMA was divided into three concentration groups (1 / 10 and 100 渭 mol / L ~ (-1);). Body sodium nitroprusside (SNP) pretreated specimen, The rats were divided into control group (10 渭 mol L ~ (-1) and SNP-ADMA group. The effects of SNP pretreatment on the electrophysiological effects of ADMA-induced left ventricular outflow tract were recorded and analyzed. Results: compared with the control group, there were no significant changes in every index of AP in 1 渭 mol L ~ (-1) ADMA group (P0.05) and 100 渭 mol L ~ (-1) ADMA group (P0.05). The spontaneous discharge frequency (RPF) and the maximum depolarization velocity (Vmax) of 0 phase were significantly decreased (P0.05), and the repolarization 50% and 90% time (APD50 and APD90) were significantly decreased (APD50 and APD90). 鏄惧欢闀，
本文编号：2135743
[Abstract]:Aim: to investigate the effect of asymmetric dimethyl arginine (ADMA), an endogenous nitric oxide synthase (NOS) inhibitor, on electrical activity in guinea pig left ventricular outflow tract (LVOT) autonomous cells, and to explore the role of ADMA in the development of ventricular outflow tract arrhythmias. Methods: the left ventricular outflow tract specimens of guinea pigs were prepared and perfused at constant temperature and at constant speed with oxygen-saturated modified Locke solution. The spontaneous slow response potential (SAP) of isolated left ventricular outflow tract of guinea pig was recorded by using standard glass microelectrode intracellular potential recording technique. The concentration-dependent effect of this potential on ADMA was observed. ADMA was divided into three concentration groups (1 / 10 and 100 渭 mol / L ~ (-1);). Body sodium nitroprusside (SNP) pretreated specimen, The rats were divided into control group (10 渭 mol L ~ (-1) and SNP-ADMA group. The effects of SNP pretreatment on the electrophysiological effects of ADMA-induced left ventricular outflow tract were recorded and analyzed. Results: compared with the control group, there were no significant changes in every index of AP in 1 渭 mol L ~ (-1) ADMA group (P0.05) and 100 渭 mol L ~ (-1) ADMA group (P0.05). The spontaneous discharge frequency (RPF) and the maximum depolarization velocity (Vmax) of 0 phase were significantly decreased (P0.05), and the repolarization 50% and 90% time (APD50 and APD90) were significantly decreased (APD50 and APD90). 鏄惧欢闀，
本文编号：2135743
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