孤立性房颤患者血浆microRNA-21水平的相关研究

发布时间：2018-07-22 16:14

【摘要】：目的：心房颤动(房颤)是最常见的心律失常,如发生在无器质性心脏病变者,则称为孤立性房颤,其相关研究较少。临床检查虽未见明显的心脏结构及运动异常,但可能已经存在轻微的结构和功能改变。MicroRNA-21 (miRNA-21)是新近发现的与心脏结构重构密切相关的小分子RNA。目前孤立性房颤与血浆miRNA-21水平关系的研究尚未见报道。本实验旨在通过研究孤立性房颤患者血浆miRNA-21水平,初步探讨血浆miRNA-21与孤立性房颤的相关性,阐述miRNA-21在孤立性房颤诊断中的价值。方法：连续收集2013年6月至2015年1月于我院心内科确诊为孤立性房颤,其中孤立性房颤组又分成阵发性房颤、持续性房颤两个亚组。健康对照组23例为来自门诊体检的健康人。所有患者均于清晨空腹静息状态下,肘静脉抽血后,提取血浆总RNA,质量鉴定后,进行逆转录,qRT-PCR技术测量血浆miRNA-21的水平。数据统计学分析采用SPSS18.0软件进行。结果：1、健康对照组血浆miRNA-21相对表达量(2-△△Ct)为1.13±0.57,而孤立性房颤组血浆miRNA-21相对表达量为1.88±0.77,差异有统计学意义(P0.01)；2、阵发性房颤和持续性房颤两个亚组,血浆miRNA-21相对表达量分别为1.49±0.61和2.19±0.76。其中阵发性房颤组miRNA-21较健康对照组有略微升高趋势,但无统计学意义(P0.05)；而持续房颤组miRNA-21明显高于健康对照组,差异有统计学意义(P0.001)；而且也高于阵发性房颤组,差异具有统计学意义(P0.01)结论：1、孤立性房颤血浆miRNA-21表达量较健康对照组明显升高,提示miRNA-21可能参与房颤的发生和维持,孤立性房颤存在心房结构重构；2、持续性房颤患者血浆miRNA-21表达量明显高于健康对照及阵发性房颤患者,‘而阵发性房颤miRNA-21表达相对于健康对照组无明显升高,提示miRNA-21的表达可能跟房颤发作的严重程度密切相关；3、miRNA-21可用作评价孤立性房颤病情严重程度及预后的客观生物标志物。
[Abstract]:Objective: atrial fibrillation (AF) is the most common arrhythmia. Although there is no obvious abnormality of cardiac structure and movement in clinical examination, there may already be slight structural and functional changes. MicroRNA-21 (miRNA-21) is a newly discovered small molecule RNA which is closely related to cardiac structural remodeling. The relationship between isolated atrial fibrillation and plasma miRNA-21 levels has not been reported. The aim of this study was to study the level of miRNA-21 in plasma of patients with isolated atrial fibrillation, and to explore the relationship between miRNA-21 and isolated atrial fibrillation, and to elucidate the value of miRNA-21 in the diagnosis of isolated atrial fibrillation. Methods: isolated atrial fibrillation was diagnosed in our hospital from June 2013 to January 2015. The isolated atrial fibrillation group was divided into two subgroups: paroxysmal atrial fibrillation and persistent atrial fibrillation. The healthy control group consisted of 23 healthy persons from outpatient examination. Plasma total RNAs were extracted from all patients at fasting resting state in the morning after cubital vein blood sampling. After quality identification, the level of miRNA-21 in plasma was measured by reverse transcription qRT-PCR. The data were analyzed by SPSS 18.0 software. Results the relative expression of miRNA-21 was 1.13 卤0.57 in healthy control group and 1.88 卤0.77 in isolated atrial fibrillation group. The difference was statistically significant (P0.01). There were two subgroups of paroxysmal atrial fibrillation and persistent atrial fibrillation. The relative expression levels of miRNA-21 in plasma were 1.49 卤0.61 and 2.19 卤0.76, respectively. The miRNA-21 in paroxysmal atrial fibrillation group was slightly higher than that in the healthy control group, but had no statistical significance (P0.05), while the miRNA-21 level in the persistent atrial fibrillation group was significantly higher than that in the healthy control group (P0.001), and was also higher than that in the paroxysmal atrial fibrillation group. The difference was statistically significant (P0.01). Conclusion the expression of miRNA-21 in isolated atrial fibrillation plasma is significantly higher than that in healthy control group (P0.01), suggesting that miRNA-21 may be involved in the occurrence and maintenance of atrial fibrillation, and isolated atrial fibrillation has atrial structural remodeling. 2. The expression of miRNA-21 in patients with persistent atrial fibrillation was significantly higher than that in healthy controls and patients with paroxysmal atrial fibrillation, but the expression of miRNA-21 in patients with paroxysmal atrial fibrillation was not significantly higher than that in healthy controls. It is suggested that miRNA-21 expression may be closely related to the severity of atrial fibrillation attack. MiRNA-21 may be used as an objective biomarker for evaluating the severity and prognosis of isolated atrial fibrillation.
【学位授予单位】：福建中医药大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R541.75

【相似文献】