血管加压素联合肾上腺素对心搏骤停大鼠心肌细胞PERK-Nrf2信号通路的影响

发布时间：2018-07-22 16:29

【摘要】：目的探讨血管加压素和肾上腺素对心搏骤停大鼠心肌细胞内质网应激PERK-Nrf2通路的作用。方法构建大鼠心搏骤停模型后,随机分为心搏骤停组(CA组)、肾上腺素治疗组(Epi组)、血管加压素治疗组(Vp组)和血管加压素联合肾上腺素治疗组(Epi+Vp组)。3个心搏骤停复苏成功1 h后留取尾静脉血,进行血清学检测。留取大鼠心肌组织行Western印迹法检测心肌组织中GRP78、PERK和Nrf2的表达水平。结果血清学检测发现,与CA组相比,Epi组、Vp组和Epi+Vp组的还原型谷胱甘肽水平明显增加。Western印迹结果显示,与CA组相比,Epi组、Vp组和Epi+Vp组心肌组织中GRP78蛋白的表达明显增加(P0.01)。同时发现与CA组相比,Epi组和Vp组PERK和Nrf2蛋白增加(P0.05),Epi+Vp组PERK和Nrf2蛋白明显增加(P0.01)。结论血管加压素联合肾上腺素通过活化PERK-Nrf2通路增加心搏骤停大鼠心肌细胞的抗氧化能力,增加心肌保护性作用。
[Abstract]:Objective to investigate the effects of vasopressin and epinephrine on ER-Nrf2 pathway in cardiac myocytes of rats with cardiac arrest. Methods the rat cardiac arrest model was established. They were randomly divided into three groups: cardiac arrest group (CA group), epinephrine group (Epi group), vasopressin group (VP group) and vasopressin combined with epinephrine group (Epi VP group). Serological tests were performed. The expression levels of GRP78PERK and Nrf2 were detected by Western blot. Results compared with CA group, the level of reduced glutathione in Epi group and Epi VP group was significantly increased. Western blot showed that the expression of GRP78 protein in myocardium of Epi group and Epi VP group was significantly higher than that of CA group (P0.01). At the same time, compared with CA group, PERK and Nrf2 protein in Epi group and VP group were increased (P0.05). PERK and Nrf2 protein increased significantly in Epi VP group (P0.01). Conclusion Vasopressin combined with epinephrine can activate PERK-Nrf2 pathway to increase the antioxidant capacity and myocardial protective effect of cardiomyocytes in rats with cardiac arrest.
【作者单位】：香港大学深圳医院;吉林大学中日联谊医院;
【基金】：吉林省卫生计生委项目(2015SZC13)
【分类号】：R541.78

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