外周血MMP-9及NF-κB在OSAHS合并高血压中的表达及其相关性研究

发布时间：2018-07-24 08:30

【摘要】：目的探讨血浆基质金属蛋白酶9(MMP-9)和核转录因子kappa B(NF-κB)在阻塞性睡眠呼吸暂停低通气综合征(OSAHS)合并高血压发病中的作用。方法选取2014年1月至6月在该院呼吸内科及神经内科行多导睡眠监测的受试者,根据睡眠监测检查结果及是否合并高血压进行分组,鼾症组23例,OSAHS组32例,OSAHS合并高血压组31例;并根据高血压级别对OSAHS合并高血压组进行亚组分型,其中OSAHS合并高血压1级组9例,2级组9例,3级组13例。分别采用酶联免疫吸附试验(ELISA)和实时荧光定量PCR的方法检测血浆MMP-9水平及单个核细胞中MMP-9和NF-κB基因的表达水平。结果OSAHS合并高血压组血浆MMP-9水平、单个核细胞MMP-9mRNA和NF-κB mRNA的表达水平均明显高于鼾症组和OSAHS组,差异有统计学意义(P0.05)。OSAHS合并高血压3级组血浆MMP-9水平、单个核细胞MMP-9 mRNA和NF-κB mRNA的表达水平均明显高于OSAHS合并高血压1级组及2级组,比较差异有统计学意义(P0.05)。血浆MMP-9水平、MMP-9mRNA及NF-κB mRNA的表达与呼吸暂停低通气指数(AHI)、氧减指数(ODI)呈正相关(P0.05),与最低血氧饱和度(LSaO2)呈负相关(P0.05)。血浆MMP-9水平与单个核细胞MMP-9mRNA的表达呈正相关(P0.05)。单个核细胞MMP-9mRNA的表达与NF-κB mRNA的表达呈正相关(P0.05)。结论血浆MMP-9水平、MMP-9mRNA及NF-κB mRNA的表达水平在OSAHS合并高血压患者中明显增高并与疾病的严重程度相关,MMP-9在OSAHS合并高血压发病中起着重要作用,可能受NF-κB通路的调节。
[Abstract]:Objective to investigate the role of matrix metalloproteinase 9 (MMP-9) and nuclear transcription factor kappa B (NF- 魏 B in the pathogenesis of obstructive sleep apnea hypopnea syndrome (OSAHS) with hypertension. Methods the subjects who received polysomnography from January to June 2014 in the department of respiratory and neurology in our hospital were divided into two groups according to the results of sleep monitoring and whether they were complicated with hypertension. There were 32 OSAHS patients in the snoring group and 31 patients in the hypertension group. According to the grade of hypertension, the subgroup classification of OSAHS combined with hypertension group was carried out, including 9 cases of OSAHS with hypertension grade 1 group, 9 cases of grade 2 hypertension group, 9 cases of grade 3 group. The levels of plasma MMP-9 and the expression of MMP-9 and NF- 魏 B genes in mononuclear cells were detected by enzyme linked immunosorbent assay (ELISA) and real-time fluorescence quantitative PCR. Results the levels of plasma MMP-9, mononuclear cell MMP-9mRNA and NF- 魏 B mRNA in OSAHS with hypertension group were significantly higher than those in snoring group and OSAHS group (P0.05). The expression levels of MMP-9 mRNA and NF- 魏 B mRNA in mononuclear cells were significantly higher than those in OSAHS with hypertension grade 1 and grade 2 groups (P0.05). The expression of MMP-9 mRNA and NF- 魏 B mRNA in plasma MMP-9 was positively correlated with apnea hypopnea index (AHI),) oxygen reduction index (ODI) (P0.05), and negatively correlated with minimum oxygen saturation (LSaO2) (P0.05). Plasma MMP-9 level was positively correlated with the expression of MMP-9mRNA in mononuclear cells (P0.05). The expression of MMP-9mRNA in mononuclear cells was positively correlated with the expression of NF- 魏 B mRNA (P0.05). Conclusion the expression of MMP-9 mRNA and NF- 魏 B mRNA in plasma MMP-9 level is significantly higher in OSAHS patients with hypertension and may play an important role in the pathogenesis of OSAHS complicated with hypertension, and may be regulated by NF- 魏 B pathway.
【作者单位】：南昌大学第二附属医院呼吸内科;
【基金】：江西省教育厅科学技术研究项目(14071)
【分类号】：R544.1;R766

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