柿叶提取物减轻大鼠心梗后心肌细胞凋亡和心室重塑相关机制研究

发布时间：2018-07-24 22:09

【摘要】：目的:探讨大鼠心梗模型下柿叶提取物对心室重塑的影响及相关机制。方法:用24只Sprague Dawley大鼠结扎左冠状动脉前降支制作心肌梗死大鼠模型,随机分为心肌梗死组(n=8)、柿叶提取物组(n=8)、假手术组(n=8)。6周后,用TUNEL法测定心肌细胞凋亡,免疫印迹法检测心肌Fas、Caspase-3蛋白含量,并测定大鼠血流动力学参数和左心室质量指数。结果:柿叶提取物组、心肌梗死组左心室质量指数分别为2.417±0.053、2.514±0.101(F=136.762,P=0.011),明显低于心肌梗死组;柿叶提取物组、心肌梗死组Fas分别是0.342±0.058、0.585±0.059(F=163.991,P=0.000);柿叶提取物组、心肌梗死组Caspase-3蛋白水平分别是0.124±0.019、0.478±0.079(F=183.553,P=0.000),明显低于心肌梗死组。柿叶提取物组、心肌梗死组心肌细胞凋亡指数分别是1.198±0.171、0.507±0.132(F=93.023,P=0.000),明显低于心肌梗死组。柿叶提取物组左心室舒张末期压(left ventricular end diastolic pressure,LVEDP)明显降低(F=347.661,P=0.000),左心室收缩压(left ventricular systolic pressure,LVSP)明显增加(F=444.949,P=0.000)。结论:心肌细胞凋亡参与心肌梗死后心室重塑过程,柿叶提取物可能通过减少凋亡相关蛋白表达抑制心肌细胞凋亡从而预防心室重塑,改善心肌梗死后心脏收缩功能。
[Abstract]:Aim: to investigate the effects of persimmon leaf extract on ventricular remodeling in rat myocardial infarction model. Methods: 24 Sprague Dawley rats were randomly divided into 4 groups: myocardial infarction group (n = 8), persimmon leaf extract group (n = 8), sham operation group (n = 8). Myocardial apoptosis was measured by TUNEL method. The contents of Fas-caspase-3 protein, hemodynamic parameters and left ventricular mass index (LVMI) were measured by Western blot. Results: the left ventricular mass index (LVMI) in the persimmon leaf extract group and myocardial infarction group was 2.417 卤0.053 卤2.514 卤0.101, respectively, which was significantly lower than that in the myocardial infarction group, and the Fas in the persimmon leaf extract group was 0.342 卤0.058, 0.585 卤0.059, respectively, and that in the persimmon leaf extract group was 0.342 卤0.058 and 0.585 卤0.059, respectively, while that in the persimmon leaf extract group was significantly lower than that in the myocardial infarction group. The level of Caspase-3 protein in myocardial infarction group was 0.124 卤0.019 卤0.478 卤0.079, respectively, which was significantly lower than that in myocardial infarction group. In persimmon leaf extract group, myocardial cell apoptosis index in myocardial infarction group was 1.198 卤0.171 卤0.507 卤0.132 (F _ (93.023) P _ (0.000), which was significantly lower than that in myocardial infarction group. In the persimmon leaf extract group, the left ventricular end-diastolic pressure (LVEDP) was significantly decreased (F _ (347.661) P _ (0.000), and the left ventricular systolic pressure (left ventricular systolic) was increased (F _ (444.949) P ~ (0.000). Conclusion: cardiomyocyte apoptosis is involved in ventricular remodeling after myocardial infarction. Persimmon leaf extract may prevent ventricular remodeling and improve cardiac systolic function by reducing the expression of apoptosis-related protein.
【作者单位】：重庆市第六人民医院心内科;重庆市急救医疗中心心内科;
【基金】：重庆市卫生计生委医学科研计划资助项目(编号:2015MSXM218)
【分类号】：R542.22

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