应用抗体芯片技术检测脓毒症血小板减少患者炎症相关细胞因子的研究
发布时间：2018-07-26 17:00
【摘要】：目的:本研究为探讨脓毒症血小板减少症患者血清中炎症相关细胞因子的表达差异,进而明确脓毒症血小板减少患者的炎症反应机制及血小板减少在脓毒症患者预后中的影响。内容:收集于2015年3月至2015年11月入住天津市第一中心医院重症监护病房(ICU)的脓毒症血小板减少患者10例,脓毒症不伴随血小板减少的患者7例,以及健康志愿者7例。以上收集的24例血清样本为研究对象,应用抗体芯片检测技术测定研究对象中34种炎症相关细胞因子的表达情况,同时观察记录上述17例脓毒症患者的预后。结果:抗体芯片技术检测血清样本中炎症相关细胞因子结果显示:脓毒症不伴血小板减少组较健康对照组细胞因子变化:促炎细胞因子:CD40L、CD40、IL-17A、IL-22、IL-6、MIP-3alpha、表达上调(fold change1.5),IL-17F、IL-21、IL-6R表达下调(fold change0.67)。抗炎细胞因子:GM-CSF表达上调(fold change1.5),IL-10表达下调(fold change0.67)。脓毒症血小板减少组较健康对照组患者,促炎细胞因子:CD30、CD40L、CD40、GITR、IL-1s RⅠ、IL-1s RⅡ、IL-17F、IL-17R、IL-1β、IL-21、IL-21R、IL-22、IL-23p19、IL-28A、IL-6、MIP-3α表达上调(fold change1.5),抗炎细胞因子:IL-12p40、IL-12p70、TGF-β1、TGF-β3、TRANCE表达上调(fold change1.5),IL-10表达下调(fold change0.67)。脓毒症血小板减少组与脓毒症不伴血小板减少组相比较,促炎细胞因子CD30、CD40L、CD40、GITR、IL-1s RⅠ、IL-1s RⅡ、IL-17F、IL-17R、IL-1β、IL-21、IL-21R、IL-23p19、IL-28A、IL-6、IL-6s R、MIP-3alpha表达上调(fold change1.5)。抗炎细胞因子:TGF-β1、TGF-β3、IL-12p40、IL-12p70、TRANCE表达上调(fold change1.5),IL-13、IL-10、GM-CSF表达下调(fold change0.67)。脓毒症血小板减少患者较脓毒症不伴血小板减少患者及健康对照者血清中大部分促炎细胞因子表达上调,只有少部分促炎细胞因子表达下调,脓毒症患者发生血小板减少提示促炎反应加剧,脓毒症伴血小板减少患者抗炎细胞因子表达紊乱。通过观察记录各组脓毒症患者的预后显示:脓毒症血小板减少组死亡率为50%,脓毒症不伴血小板减少组死亡率为28.6%。脓毒症伴血小板减少患者死亡率较脓毒症不伴血小板减少患者死亡率较高。结论:脓毒症促炎及抗炎免疫失调,以促炎反应为主,脓毒症伴血小板减少促炎反应进一步加剧,抗炎反应属免疫抑制状态。血小板活化参与脓毒症的发病,脓毒症伴血小板减少血小板活化进一步增加。脓毒症伴血小板减少死亡风险增高。
[Abstract]:Objective: to investigate the expression of inflammatory cytokines in serum of patients with sepsis thrombocytopenia, and to clarify the mechanism of inflammatory response and the influence of thrombocytopenia on the prognosis of patients with sepsis. Contents: ten patients with sepsis, 7 patients with sepsis without thrombocytopenia and 7 healthy volunteers who were admitted to (ICU) of Tianjin first Central Hospital from March 2015 to November 2015 were collected. 24 serum samples collected above were used to detect the expression of 34 inflammatory cytokines and the prognosis of 17 patients with sepsis were observed. Results: the results of detection of inflammatory cytokines in serum samples by antibody chip technique showed that the cytokines in sepsis without thrombocytopenia group were higher than those in healthy control group. The cytokines of proinflammatory cytokines: 1 / CD40L / CD40 / IL-17A ~ + IL-22 / IL-6MIP-3alpha were up-regulated (fold change1.5) / IL-17FU / IL-21 / IL-6R expression (fold change0.67). Anti-inflammatory cytokine: GM-CSF up-regulated (fold change1.5) down-regulated IL-10 expression (fold change0.67). In sepsis thrombocytopenia group, the inflammatory cytokines CD40L-, CD40TITR+, IL-1s R 鈪，
本文编号：2146680
[Abstract]:Objective: to investigate the expression of inflammatory cytokines in serum of patients with sepsis thrombocytopenia, and to clarify the mechanism of inflammatory response and the influence of thrombocytopenia on the prognosis of patients with sepsis. Contents: ten patients with sepsis, 7 patients with sepsis without thrombocytopenia and 7 healthy volunteers who were admitted to (ICU) of Tianjin first Central Hospital from March 2015 to November 2015 were collected. 24 serum samples collected above were used to detect the expression of 34 inflammatory cytokines and the prognosis of 17 patients with sepsis were observed. Results: the results of detection of inflammatory cytokines in serum samples by antibody chip technique showed that the cytokines in sepsis without thrombocytopenia group were higher than those in healthy control group. The cytokines of proinflammatory cytokines: 1 / CD40L / CD40 / IL-17A ~ + IL-22 / IL-6MIP-3alpha were up-regulated (fold change1.5) / IL-17FU / IL-21 / IL-6R expression (fold change0.67). Anti-inflammatory cytokine: GM-CSF up-regulated (fold change1.5) down-regulated IL-10 expression (fold change0.67). In sepsis thrombocytopenia group, the inflammatory cytokines CD40L-, CD40TITR+, IL-1s R 鈪，
本文编号：2146680
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